Bakonyi Tamás, Hubálek Zdenek, Rudolf Ivo, Nowotny Norbert
University of Veterinary Medicine, A-1210 Vienna, Austria.
Emerg Infect Dis. 2005 Feb;11(2):225-31. doi: 10.3201/eid1102.041028.
A flavivirus (strain 97-103) was isolated from Culex pipens mosquitoes in 1997 following floods in South Moravia, Czech Republic. The strain exhibited close antigenic relationship to West Nile virus (WNV) prototype strain Eg-101 in a cross-neutralization test. In this study, mouse pathogenicity characteristics and the complete nucleotide and putative amino acid sequences of isolate 97-103, named Rabensburg virus (RabV) after a nearby Austrian city, were determined. RabV shares only 75%-77% nucleotide identity and 89%-90% amino acid identity with representative strains of WNV lineages 1 and 2. Another RabV strain (99-222) was isolated in the same location 2 years later; it showed >99% nucleotide identity to strain 97-103. Phylogenetic analyses of RabV, WNV strains, and other members of the Japanese encephalitis virus (JEV) complex clearly demonstrated that RabV is either a new (third) lineage of WNV or a novel flavivirus of the JEV group.
1997年捷克共和国南摩拉维亚发生洪水后，从尖音库蚊中分离出一种黄病毒（97 - 103株）。在交叉中和试验中，该毒株与西尼罗河病毒（WNV）原型毒株Eg - 101表现出密切的抗原关系。在本研究中，确定了97 - 103分离株的小鼠致病性特征以及完整的核苷酸和推测的氨基酸序列，该分离株以附近的奥地利城市命名为拉本斯堡病毒（RabV）。RabV与WNV第1和第2谱系的代表性毒株仅具有75% - 77%的核苷酸同一性和89% - 90%的氨基酸同一性。两年后在同一地点分离出另一种RabV毒株（99 - 222）；它与97 - 103毒株的核苷酸同一性>99%。对RabV、WNV毒株以及日本脑炎病毒（JEV）复合体其他成员的系统发育分析清楚地表明，RabV要么是WNV的一个新的（第三个）谱系，要么是JEV组的一种新型黄病毒。
Zotero 插件