免耕法：一种应用于反向遗传学的新方法。

TILLING without a plough: a new method with applications for reverse genetics.

作者信息

Gilchrist Erin J, Haughn George W

机构信息

Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, British Colombia, V6T 1Z4, Canada.

出版信息

Curr Opin Plant Biol. 2005 Apr;8(2):211-5. doi: 10.1016/j.pbi.2005.01.004.

DOI:10.1016/j.pbi.2005.01.004

PMID:15753003

Abstract

TILLING (Targeting Induced Local Lesions IN Genomes) is a powerful reverse genetic technique that employs a mismatch-specific endonuclease to detect induced or natural DNA polymorphisms. Its advantages over other reverse genetic techniques include its applicability to virtually any organism, its facility for high-throughput and its independence of genome size, reproductive system or generation time. TILLING is currently being used for the detection of both induced and natural variation in several plant species.

摘要

定向诱导基因组局部突变技术（TILLING）是一种强大的反向遗传学技术，它利用错配特异性核酸内切酶来检测诱导产生的或天然的DNA多态性。与其他反向遗传学技术相比，它的优势包括几乎适用于任何生物体、易于进行高通量操作，并且不受基因组大小、生殖系统或世代时间的影响。目前，定向诱导基因组局部突变技术正被用于检测多种植物物种中的诱导变异和天然变异。