Razzaq Abdul, Zafar Muhammad Mubashar, Ali Arfan, Hafeez Abdul, Sharif Faiza, Guan Xueing, Deng Xiaoying, Pengtao Li, Shi Yuzhen, Haroon Muhammad, Gong Wankui, Ren Maozhi, Yuan Youlu
State Key Laboratory of Cotton Biology, The Ministry of Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang, China.
Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministry of Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang, China.
Front Genet. 2022 Mar 24;12:642595. doi: 10.3389/fgene.2021.642595. eCollection 2021.
Lack of precise information about the candidate genes involved in a complex quantitative trait is a major obstacle in the cotton fiber quality improvement, and thus, overall genetic gain in conventional phenotypic selection is low. Recent molecular interventions and advancements in genome sequencing have led to the development of high-throughput molecular markers, quantitative trait locus (QTL) fine mapping, and single nucleotide polymorphisms (SNPs). These advanced tools have resolved the existing bottlenecks in trait-specific breeding. This review demonstrates the significance of chromosomes 3, 7, 9, 11, and 12 of sub-genomes A and D carrying candidate genes for fiber quality. However, chromosome 7 carrying SNPs for stable and potent QTLs related to fiber quality provides great insights for fiber quality-targeted research. This information can be validated by marker-assisted selection (MAS) and transgene in and subsequently in cotton.
缺乏与复杂数量性状相关的候选基因的确切信息是棉花纤维品质改良的主要障碍,因此,传统表型选择中的总体遗传增益较低。最近的分子干预和基因组测序进展导致了高通量分子标记、数量性状位点(QTL)精细定位和单核苷酸多态性(SNP)的发展。这些先进工具解决了特定性状育种中现有的瓶颈问题。本综述阐述了A和D亚基因组的3号、7号、9号、11号和12号染色体携带纤维品质候选基因的重要性。然而,携带与纤维品质相关的稳定且有效的QTL的SNP的7号染色体为针对纤维品质的研究提供了重要见解。这些信息可以通过标记辅助选择(MAS)和转基因在拟南芥中进行验证,随后在棉花中进行验证。