基于Ty1-copia反转录转座子的腰果（Anacardium occidentale L.）SSAP标记开发
Ty1-copia retrotransposon-based SSAP marker development in cashew (Anacardium occidentale L.).
作者信息
Syed N H, Sureshsundar S, Wilkinson M J, Bhau B S, Cavalcanti J J V, Flavell A J
机构信息
Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee, DD2 5DA, UK.
出版信息
Theor Appl Genet. 2005 May;110(7):1195-202. doi: 10.1007/s00122-005-1948-1. Epub 2005 Mar 11.
The most popular retrotransposon-based molecular marker system in use at the present time is the sequence-specific amplification polymorphism (SSAP) system . This system exploits the insertional polymorphism of long terminal repeat (LTR) retrotransposons around the genome. Because the LTR sequence is used to design primers for this method, its successful application requires sequence information from the terminal region of the mobile elements . In this study, two LTR sequences were isolated from the cashew genome and used successfully to develop SSAP marker systems. These were shown to have higher levels of polymorphism than amplified fragment length polymorphic markers for this species.
目前使用的最流行的基于反转录转座子的分子标记系统是序列特异性扩增多态性（SSAP）系统。该系统利用基因组周围长末端重复（LTR）反转录转座子的插入多态性。由于该方法使用LTR序列设计引物，其成功应用需要来自移动元件末端区域的序列信息。在本研究中，从腰果基因组中分离出两个LTR序列，并成功用于开发SSAP标记系统。结果表明，对于该物种，这些标记系统比扩增片段长度多态性标记具有更高水平的多态性。
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