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饮用水中类鼻疽伯克霍尔德菌的消毒

Disinfection of Burkholderia pseudomallei in potable water.

作者信息

Howard Kay, Inglis Timothy J J

机构信息

Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Locked Bag 2009, Nedlands WA 6909, Australia.

出版信息

Water Res. 2005 Mar;39(6):1085-92. doi: 10.1016/j.watres.2004.12.028.

Abstract

The effect of chlorine, monochloramine and UV disinfection on the water-borne pathogen Burkholderia pseudomallei was assessed. Persistence of B. pseudomallei was verified by MPN involving a one-step recovery procedure. Chlorine proved the most effective disinfectant with a 99.99% reduction of a 10(6) CFU/mL pure bacterial culture followed by 99.9% reduction by monochloramine and 99% reduction by UV. Co-culture of B. pseudomallei with Acanthamoeba astronyxis was found to greatly enhance survival of B. pseudomallei in the presence of all disinfecting agents tested. For example, when amoebae were present 100 times more monochloramine was required to maintain the disinfectant efficacy. Given the results obtained from these co-culture experiments, more research is needed to investigate the role of amoeba and biofilms in survival of B. pseudomallei in potable water.

摘要

评估了氯、一氯胺和紫外线消毒对水源性病原体类鼻疽伯克霍尔德菌的影响。通过采用一步恢复程序的最大可能数法验证了类鼻疽伯克霍尔德菌的存活情况。结果表明,氯是最有效的消毒剂,可使10(6) CFU/mL的纯细菌培养物减少99.99%,其次是一氯胺使细菌减少99.9%,紫外线使细菌减少99%。研究发现,在所有测试的消毒剂存在的情况下,类鼻疽伯克霍尔德菌与星状棘阿米巴的共培养大大提高了类鼻疽伯克霍尔德菌的存活率。例如,当存在变形虫时,需要100倍以上的一氯胺才能维持消毒效果。鉴于这些共培养实验的结果,需要开展更多研究来调查变形虫和生物膜在饮用水中类鼻疽伯克霍尔德菌存活中的作用。

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