Pang Xiaoyan, Ding Dezhong, Wei Guifang, Zhang Meiling, Wang Linghua, Zhao Liping
Laboratory of Molecular Microbial Ecology and Ecogenomics, Department of Bioscience and Biotechnology, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
J Microbiol Methods. 2005 Jun;61(3):413-7. doi: 10.1016/j.mimet.2004.12.012. Epub 2005 Jan 22.
A group-specific PCR-based temperature gradient gel electrophoresis (TGGE) method was developed to study the population composition of genus Bacteroides in human gut. Highly reproducible and well-separated bands in TGGE fingerprints of ten unrelated human fecal samples showed complex and host-specific Bacteroides species composition. Dynamic monitoring over 22 months of samples from one healthy 10-year-old boy indicated a relatively stable population profile of Bacteroides. The species identity of each band in TGGE gel of this boy was also resolved via comigration analysis of sequenced inserts in a Bacteroides group-specific clone library. This work provides a rapid and effective technique for analyzing the species composition of Bacteroides in human gut.
开发了一种基于群体特异性聚合酶链反应的温度梯度凝胶电泳（TGGE）方法，用于研究人类肠道中拟杆菌属的菌群组成。十个无亲缘关系的人类粪便样本的TGGE指纹图谱中具有高度可重复性且条带分离良好，显示出复杂且宿主特异性的拟杆菌种类组成。对一名健康10岁男孩的样本进行了22个月的动态监测，结果表明拟杆菌的群体概况相对稳定。通过对拟杆菌属特异性克隆文库中测序插入片段的共迁移分析，也确定了该男孩TGGE凝胶中每条带的物种身份。这项工作为分析人类肠道中拟杆菌的种类组成提供了一种快速有效的技术。
