Gálvez Marina, Martín-Cordero Carmen, Houghton Peter J, Ayuso María Jesús
Departamento de Farmacología, Facultad de Farmacia, University of Seville, C/P. García González 2, 41012 Seville, Spain.
J Agric Food Chem. 2005 Mar 23;53(6):1927-33. doi: 10.1021/jf048076s.
Antioxidative activities of methanol extracts from five Plantago species (P. afra, P. coronopus, P. lagopus, P. lanceolata, and P. serraria) were characterized by the DPPH scavenging test and the inhibition of Fe2+/ascorbate-induced lipid peroxidation on bovine brain liposomes. All extracts showed antioxidant activity in both methods. Whereas P. serraria exhibited the strongest activity as a DPPH scavenger, P. lanceolata and P. serraria were found to be the most active in the lipid peroxidation inhibition assay. The extracts were investigated regarding their composition by different colorimetric techniques, such as the content of total phenolic compounds by the Folin-Ciocalteu assay, flavonoids by AlCl3 reagent, phenylpropanoid glycosides (PPGs) by Arnow reagent, and iridoids by Trim-Hill assay. A high correlation was found between the scavenging potency and the total phenolic and phenylpropanoid content of the extracts but not between the lipid peroxidation potency and the extract composition. P. serraria is presented as a possible new source of natural antioxidants.
通过DPPH清除试验以及对牛脑脂质体上Fe2+/抗坏血酸诱导的脂质过氧化的抑制作用，对五种车前草属植物（非洲车前草、海滨车前草、兔耳车前草、披针叶车前草和锯齿车前草）甲醇提取物的抗氧化活性进行了表征。在这两种方法中，所有提取物均表现出抗氧化活性。虽然锯齿车前草作为DPPH清除剂表现出最强的活性，但在脂质过氧化抑制试验中，披针叶车前草和锯齿车前草被发现活性最高。通过不同的比色技术对提取物的成分进行了研究，例如通过福林-西奥尔特法测定总酚类化合物的含量，通过三氯化铝试剂测定黄酮类化合物的含量，通过阿诺试剂测定苯丙素苷（PPGs）的含量，以及通过特里姆-希尔法测定环烯醚萜类化合物的含量。发现提取物的清除能力与总酚和苯丙素含量之间存在高度相关性，但脂质过氧化能力与提取物成分之间不存在相关性。锯齿车前草被认为是天然抗氧化剂的一个可能的新来源。
