过氧化氢的细胞内信使功能及其过氧化物酶体增殖物激活受体的调节

Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins.

作者信息

Rhee Sue Goo, Kang Sang Won, Jeong Woojin, Chang Tong-Shin, Yang Kap-Seok, Woo Hyun Ae

机构信息

Laboratory of Cell Signaling, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Curr Opin Cell Biol. 2005 Apr;17(2):183-9. doi: 10.1016/j.ceb.2005.02.004.

DOI:10.1016/j.ceb.2005.02.004

PMID:15780595

Abstract

Hydrogen peroxide (H2O2) accumulates transiently in various cell types stimulated with peptide growth factors and participates in receptor signaling by oxidizing the essential cysteine residues of protein tyrosine phosphatases and the lipid phosphatase PTEN. The reversible inactivation of these phosphatases by H2O2 is likely required to prevent futile cycles of phosphorylation-dephosphorylation of proteins and phosphoinositides. The accumulation of H2O2 is possible even in the presence of large amounts of the antioxidant enzymes peroxiredoxin I and II in the cytosol, probably because of a built-in mechanism of peroxiredoxin inactivation that is mediated by H2O2 and reversed by an ATP-dependent reduction reaction catalyzed by sulfiredoxin.

摘要

过氧化氢（H₂O₂）在受到肽生长因子刺激的各种细胞类型中短暂积累，并通过氧化蛋白质酪氨酸磷酸酶和脂质磷酸酶PTEN的必需半胱氨酸残基参与受体信号传导。H₂O₂对这些磷酸酶的可逆失活可能是为了防止蛋白质和磷酸肌醇的磷酸化-去磷酸化无效循环。即使在细胞质中存在大量抗氧化酶过氧化物酶I和II的情况下，H₂O₂仍有可能积累，这可能是由于过氧化物酶失活的内在机制，该机制由H₂O₂介导，并由硫氧还蛋白催化的ATP依赖性还原反应逆转。