Ivanova Emilia, Toshkova Reneta, Serkedjieva Julia
Institute of Microbiology, Bulgarian Academy of Sciences, 26 Acad. G. Bonchev St., Sofia 1113, Bulgaria.
Microbes Infect. 2005 Mar;7(3):391-8. doi: 10.1016/j.micinf.2004.11.013. Epub 2005 Mar 16.
Influenza infection was induced in white ICR mice by intranasal (i.n.) inoculation of the virus A/Aichi/2/68 (H3N2). The number, migration and phagocyte indices of alveolar and peritoneal macrophages (pMØ) and of blood polymorphonuclear leukocytes (PMNs), as well as the inhibition of the PMN adherence in the presence of a specific antigen were followed for 9 days after infection. The effect of the i.n. application of a polyphenol-rich extract, designated as polyphenolic complex (PC), isolated from the medicinal plant Geranium sanguineum L., on the inspected immune parameters was studied in parallel with the virological parameters of the infection, e.g. rate of mortality, mean survival time (MST), infectious lung virus titre and consolidation of the lungs. It was found that the application of PC induced a continuous 2- to 2.5-fold rise in the number of both peritoneal and alveolar macrophages (aMØ) in the infected and healthy controls. The migration of both peritoneal and aMØ increased 1.5- to 2-fold in the group of infected PC-treated animals and four to fivefold in the control group, the maximum being on day 9. PC stimulated phagocyte activities of blood PMNs in both infected and healthy mice. The leukocyte adherence inhibition (LAI) index decreased in the infected and PC-treated animals. The restoration of the suppressed functions of phagocytes in influenza virus-infected mice (VIM) was consistent with a prolongation of MST and reduction in mortality rate, infectious virus titre and lung consolidation. The immunoenhancing properties of PC apparently contribute to the overall protective effect of the plant preparation in the lethal murine experimental influenza A/Aichi infection.
通过鼻内（i.n.）接种A/爱知/2/68（H3N2）病毒，在白色ICR小鼠中诱发流感感染。在感染后的9天内，跟踪观察肺泡巨噬细胞和腹腔巨噬细胞（pMØ）以及血液多形核白细胞（PMN）的数量、迁移和吞噬指数，以及在存在特异性抗原的情况下PMN黏附的抑制情况。同时，研究了从药用植物血红老鹳草中分离出的富含多酚的提取物（称为多酚复合物，PC）经鼻内给药对所检测的免疫参数的影响，并与感染的病毒学参数进行了比较，如死亡率、平均存活时间（MST）、感染性肺病毒滴度和肺部实变情况。结果发现，PC的应用使感染组和健康对照组的腹腔巨噬细胞和肺泡巨噬细胞（aMØ）数量持续增加2至2.5倍。在感染PC处理组动物中，腹腔巨噬细胞和肺泡巨噬细胞的迁移增加了1.5至2倍，而在对照组中增加了4至5倍，在第9天达到最大值。PC刺激了感染和健康小鼠血液中PMN的吞噬活性。在感染并接受PC处理的动物中，白细胞黏附抑制（LAI）指数降低。流感病毒感染小鼠（VIM）中吞噬细胞受抑制功能的恢复与MST延长、死亡率、感染性病毒滴度和肺部实变降低相一致。PC的免疫增强特性显然有助于该植物制剂对致死性小鼠实验性A/爱知流感感染的总体保护作用。
