Serkedjieva Julia, Toshkova Reneta, Antonova-Nikolova Stefka, Stefanova Tsvetanka, Teodosieva Ani, Ivanova Iskra
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Antivir Chem Chemother. 2007;18(2):75-82. doi: 10.1177/095632020701800203.
Influenza infection was induced in white mice by intranasal inoculation of the virus A/Aichi/2/68 (H3N2). The lung protease and the protease-inhibitory activities were followed for 9 days after infection. The intranasal application of a polyphenol-rich extract (PC) isolated from Geranium sanguineum L. induced a continuous rise in the anti-protease activity but did not cause substantial changes in the lung protease activity of healthy mice. Influenza virus infection triggered a slight reduction in protease activity in the lungs at 5 and 48 h post infection (p.i.) and a marked increase at 24 h and 6 day p.i.. Protease inhibition in the lungs was reduced at 24 and 48 h p.i. and an increase was observed at 5 h and 6 and 9 days p.i.. PC treatment brought both activities to normal levels. The restoration of the examined parameters was consistent with a prolongation of mean survival time and reduction of mortality rate, infectious virus titre and lung consolidation. PC reinstated superoxide production by alveolar macrophages and increased their number in virus-infected mice. The favourable effect on the protease and the protease-inhibitory activities in the lungs of influenza-virus-infected mice apparently contributes to the overall protective effect of PC in the murine experimental influenza A/Aichi infection. The antiviral effect of the individual constituents was evaluated.
通过鼻内接种A/爱知/2/68（H3N2）病毒在小白鼠中诱发流感感染。在感染后9天跟踪观察肺蛋白酶和蛋白酶抑制活性。从血红老鹳草中分离出的富含多酚的提取物（PC）经鼻内给药后，健康小鼠的抗蛋白酶活性持续上升，但肺蛋白酶活性未发生实质性变化。流感病毒感染在感染后5小时和48小时使肺中的蛋白酶活性略有降低，在感染后24小时和6天则显著增加。在感染后24小时和48小时，肺中的蛋白酶抑制作用降低，在感染后5小时以及6天和9天观察到蛋白酶抑制作用增加。PC处理使这两种活性恢复到正常水平。所检测参数的恢复与平均存活时间的延长以及死亡率、感染性病毒滴度和肺实变的降低相一致。PC恢复了病毒感染小鼠肺泡巨噬细胞的超氧化物产生并增加了其数量。对流感病毒感染小鼠肺中蛋白酶和蛋白酶抑制活性的有利影响显然有助于PC在小鼠实验性A/爱知流感感染中的总体保护作用。评估了各个成分的抗病毒效果。
