Institute of Microbiology, Bulgarian Academy of Sciences, 26, Acad. G. Bonchev St., 1113 Sofia, Bulgaria.
Z Naturforsch C J Biosci. 2010 May-Jun;65(5-6):419-28. doi: 10.1515/znc-2010-5-616.
The combined protective effect of a polyphenol-rich extract, isolated from Geranium sanguineum L. (PC), and a novel naturally glycosylated Cu/Zn-containing superoxide dismutase, produced from the fungal strain Humicula lutea 103 (HL-SOD), in the experimental influenza A virus infection (EIVI) in mice, induced with the virus A/Aichi/2/68 (H3N2), was investigated. The combined application of HL-SOD and PC in doses, which by themselves do not defend significantly mice in EIVI, resulted in a synergistically increased protection, determined on the basis of protective indices and amelioration of lung injury. Lung weights and consolidation as well as infectious lung virus titers were all decreased significantly parallel to the reduction of the mortality rates; lung indices were raised. The excessive production of reactive oxygen species (ROS) by alveolar macrophages (aMphi) as well as the elevated levels of the lung antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), induced by EIVI, were brought to normal. For comparative reasons the combined protective effect of PC and vitamin C was investigated. The obtained results support the combined use of antioxidants for the treatment of influenza virus infection and in general indicate the beneficial protective role of combinations of viral inhibitors of natural origin with diverse modes of action.
研究了从天竺葵（Geranium sanguineum L.）中分离得到的富含多酚的提取物（PC）与新型天然糖基化铜锌超氧化物歧化酶（HL-SOD）联合应用对流感病毒 A/Aichi/2/68（H3N2）诱导的实验性流感病毒感染（EIVI）的保护作用。HL-SOD 和 PC 的联合应用剂量本身不能显著保护 EIVI 中的小鼠，但在保护指数和肺损伤改善方面表现出协同的保护作用。肺重和肺实变以及感染性肺病毒滴度均显著降低，死亡率也相应降低；肺指数升高。肺泡巨噬细胞（aMphi）产生的过量活性氧（ROS）以及由 EIVI 引起的肺抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平升高均恢复正常。为了进行比较，还研究了 PC 和维生素 C 的联合保护作用。所得结果支持联合使用抗氧化剂治疗流感病毒感染，并普遍表明具有不同作用机制的天然来源的病毒抑制剂联合使用具有有益的保护作用。
