Barman T K, Sharma V D, Kumar Subodh
Department of Veterinary Microbiology, College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263 145, India.
Indian J Exp Biol. 2005 Feb;43(2):163-6.
An attempt was made to evaluate the protective efficacy of maternal antibodies in chicks against salmonellosis. Layer chicks ageing 21 days were individually vaccinated with 100 microg of Salmonella enterica subspecies enterica serovar Weltevreden (BM 1643) toxoid adjuvanted with vitamin E subcutaneously. After 90 days of the primary vaccination the birds were given booster dose of the vaccine. The saline extract of the yolk of eggs laid by the vaccinated birds yielded agglutination and ELISA titres ranging from 43.2 +/- 5.33 to 75.2 +/- 6.26 and 4.987 x 10(3) +/- 0.54 to 5.89x103 +/- 0.56, respectively. Sera of chicks hatched from eggs laid by the vaccinated layers were also subjected to agglutination and ELISA. Agglutination and ELISA titres on the 5th day--post hatching (dph) were 21.6 +/- 1.75 and 4.025 x 10(2) +/- 0.59, while on the 10th dph titers were 13.6 +/- 1.65 and 1.21 x 10(2) +/- 0.60, respectively. It was also observed that only one out of 6 chicks died when challenged with 2 x 10(9) CFU of S. serovar Gallirarum at the age of 7 days showing 83.33% protection. Thus it can be concluded that passive immunity confided by Salmonella enterica subspecies enterica serovar Weltevreden (BM 1643) toxoid can protect chicks against salmonellosis during their early days of life.
研究人员尝试评估母源抗体对雏鸡沙门氏菌病的保护效果。选取21日龄的蛋鸡雏，每只皮下接种100微克肠炎沙门氏菌肠炎亚种韦尔泰弗里登血清型（BM 1643）类毒素，并佐以维生素E。初次接种90天后，给鸡群进行疫苗加强免疫。接种疫苗的蛋鸡所产鸡蛋的蛋黄盐水提取物的凝集效价和ELISA效价分别为43.2±5.33至75.2±6.26以及4.987×10³±0.54至5.89×10³±0.56。对接种蛋鸡所产鸡蛋孵化出的雏鸡血清也进行了凝集试验和ELISA检测。孵化后第5天（dph）的凝集效价和ELISA效价分别为21.6±1.75和4.025×10²±0.59，而在第10 dph时效价分别为13.6±1.65和1.21×10²±0.60。还观察到，6只雏鸡中有1只在7日龄时受到2×10⁹CFU鸡白痢沙门氏菌攻击后死亡，保护率为83.33%。因此可以得出结论，肠炎沙门氏菌肠炎亚种韦尔泰弗里登血清型（BM 1643）类毒素赋予的被动免疫可在雏鸡生命早期保护其免受沙门氏菌病感染。
