来自玻利维亚和秘鲁健康儿童粪便中大肠杆菌的获得性磺胺耐药基因。
Acquired sulphonamide resistance genes in faecal Escherichia coli from healthy children in Bolivia and Peru.
作者信息
Infante Berónica, Grape Malin, Larsson Mattias, Kristiansson Charlotte, Pallecchi Lucia, Rossolini Gian Maria, Kronvall Göran
机构信息
Microbiology and Tumor Biology Centre (MTC), Clinical Microbiology, Karolinska Institute, SE-17176 Stockholm, Sweden.
出版信息
Int J Antimicrob Agents. 2005 Apr;25(4):308-12. doi: 10.1016/j.ijantimicag.2004.12.004.
Antimicrobial resistance and sulphonamide resistance determinants were studied in 20 co-trimoxazole resistant Escherichia coli in faecal samples from healthy children in Bolivia and Peru. Methods used were disc diffusion susceptibility tests, PCR, sequence analysis and plasmid conjugation assays. All isolates but one were resistant to at least two different classes of antimicrobials; 19 isolates also carried at least one sul-gene. The most frequent gene was sul2 followed by sul1 and sul3, which was detected in one isolate. This is the first observation of sul3 on the American continent. In conclusion, the high prevalence of sul-genes in this material of faecal commensal E. coli isolates points to a potential role of the faecal flora in the emergence and spread of antimicrobial resistance.
对来自玻利维亚和秘鲁健康儿童粪便样本中的20株复方新诺明耐药大肠杆菌进行了抗菌药物耐药性和磺胺耐药性决定因素研究。所采用的方法包括纸片扩散药敏试验、聚合酶链反应（PCR）、序列分析和质粒接合试验。除1株外，所有分离株均对至少两类不同抗菌药物耐药；19株分离株还携带至少一个磺胺基因。最常见的基因是sul2，其次是sul1，sul3在1株分离株中被检测到。这是在美洲大陆首次发现sul3。总之，粪便共生大肠杆菌分离株材料中磺胺基因的高流行率表明粪便菌群在抗菌药物耐药性的出现和传播中可能发挥作用。
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