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在果蝇卵子发生过程中,鱿鱼蛋白对于osk基因mRNA高效地定位到卵子后端是必需的。

Squid is required for efficient posterior localization of oskar mRNA during Drosophila oogenesis.

作者信息

Norvell Amanda, Debec Alain, Finch Daniel, Gibson Lisa, Thoma Brandi

机构信息

Department of Biology, The College of New Jersey, PO Box 7718, Ewing, NJ 08628-0718, USA.

出版信息

Dev Genes Evol. 2005 Jul;215(7):340-9. doi: 10.1007/s00427-005-0480-2. Epub 2005 Mar 25.

Abstract

The nuclear-cytoplasmic shuttling heterogeneous nuclear RNA-binding protein (hnRNP) Squid (Sqd) is required during Drosophila melanogaster oogenesis, where it plays a critical role in the regulation of the TGFalpha-like molecule Gurken (Grk). Three Sqd isoforms have been described, SqdA, S and B, and two of these, SqdA and SqdS, differentially function in grk mRNA nuclear export, cytoplasmic transport and translational control during oogenesis. Here, we report that Sqd is also required for the regulation of oskar (osk) mRNA, functioning in the cytoplasmic localization of the osk transcript. In oocytes from sqd females, osk mRNA is not efficiently localized to the posterior pole, but rather accumulates at the anterior cortex. Furthermore, anterior patterning defects observed in embryos from sqd females expressing only the SqdS protein isoform suggest that Sqd may also play a role in the translational regulation of the mislocalized osk mRNA. These findings provide additional support for models of mRNA regulation in which cytoplasmic events, such as localization and translational regulation, are coupled. These results also place Sqd among an emerging class of proteins, including such other members as Bruno (Bru) and Hrb27C/Hrp48, which function in multiple aspects of both grk and osk mRNA regulation during Drosophila oogenesis.

摘要

核质穿梭异质核RNA结合蛋白(hnRNP)鱿鱼蛋白(Sqd)在黑腹果蝇卵子发生过程中是必需的,它在TGFα样分子 Gurken(Grk)的调控中起关键作用。已描述了三种Sqd异构体,即SqdA、S和B,其中两种,SqdA和SqdS,在卵子发生过程中对grk mRNA的核输出、细胞质运输和翻译控制具有不同的功能。在此,我们报告Sqd对于oskar(osk)mRNA的调控也是必需的,它在osk转录本的细胞质定位中发挥作用。在sqd雌性果蝇的卵母细胞中,osk mRNA不能有效地定位于后极,而是在前皮质积累。此外,在仅表达SqdS蛋白异构体的sqd雌性果蝇的胚胎中观察到的前部模式缺陷表明,Sqd可能在错误定位的osk mRNA的翻译调控中也发挥作用。这些发现为mRNA调控模型提供了额外支持,在该模型中,细胞质事件,如定位和翻译调控,是相互关联的。这些结果还将Sqd置于一类新兴的蛋白质中,包括Bruno(Bru)和Hrb27C/Hrp48等其他成员,它们在果蝇卵子发生过程中对grk和osk mRNA调控的多个方面发挥作用。

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