Gupta Richa, Flora Swaran J S
Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474-002, India.
Phytother Res. 2005 Jan;19(1):23-8. doi: 10.1002/ptr.1560.
Concomitant oral supplementation of Aloe vera, (1, 2 or 5% w[sol ]v in drinking water) during arsenic exposure (0.2 mg[sol ]kg, intraperitoneally, once daily for 3 weeks) was investigated in rats for its protective value. Animals exposed to arsenic (III) showed a significant inhibition of delta-aminolevulinic acid dehydratase (ALAD) activity, a marginal decrease in glutathione (GSH) and an increase in zinc protoporphyrin (ZPP) level in blood. White blood corpuscles (WBC) level decreased while most of the other clinical blood parameters like red blood cells count, haemoglobin, MCV, MCH, MCHC ratio and platelet number, etc. remained unaltered on arsenic exposure. Hepatic reduced GSH, oxidized glutathione (GSSG) level remained unaltered, thiobarbituric acid reactive substance (TBARS) level increased significantly while the activity of alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and catalase decreased on arsenic exposure. Renal GSH contents decreased while superoxide dismutase (SOD) activity decreased significantly on arsenic exposure. Concomitant administration of Aloe vera had remarkable protective action on inhibited blood ALAD activity and restored blood GSH level while most of the other blood biochemical parameters remained unchanged on Aloe vera supplementation. Interestingly, most of hepatic biochemical variables indicative of oxidative stress showed protection; no effect of Aloe vera on blood and liver arsenic concentration was noted. Also, no effect of Aloe vera on most of the altered renal biochemical parameters were noticed. The results thus lead us to conclude that simultaneous supplementation of Aloe vera protects against arsenic induced oxidative stress but does not influence the arsenic concentration in these organs.
在大鼠中研究了在砷暴露（0.2毫克/千克，腹腔注射，每日一次，持续3周）期间同时口服补充芦荟（饮用水中1%、2%或5%重量/体积）的保护作用。暴露于砷（III）的动物显示δ-氨基乙酰丙酸脱水酶（ALAD）活性显著抑制，谷胱甘肽（GSH）略有下降，血液中锌原卟啉（ZPP）水平升高。白细胞（WBC）水平下降，而大多数其他临床血液参数如红细胞计数、血红蛋白、平均红细胞体积、平均红细胞血红蛋白含量、平均红细胞血红蛋白浓度比值和血小板数量等在砷暴露后保持不变。肝脏中还原型谷胱甘肽、氧化型谷胱甘肽（GSSG）水平保持不变，硫代巴比妥酸反应物质（TBARS）水平显著升高，而碱性磷酸酶（ALP）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和过氧化氢酶的活性在砷暴露后降低。肾脏中GSH含量下降，而超氧化物歧化酶（SOD）活性在砷暴露后显著降低。同时给予芦荟对受抑制的血液ALAD活性有显著保护作用，并恢复了血液GSH水平，而补充芦荟后大多数其他血液生化参数保持不变。有趣的是，大多数指示氧化应激的肝脏生化变量显示出保护作用；未观察到芦荟对血液和肝脏中砷浓度的影响。此外，未注意到芦荟对大多数改变的肾脏生化参数有影响。因此，结果使我们得出结论，同时补充芦荟可预防砷诱导的氧化应激，但不影响这些器官中的砷浓度。
