Biju S S, Ahuja A, Khar R K, Chaudhry R
Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, India.
Pharmazie. 2005 Mar;60(3):208-11.
The effect of pH on the antimicrobial activity of Melaleuca alternifolia essential oil formulations was studied. Microemulsions, liposomal dispersions, multiple emulsions and a colloidal bed of sterile clay were formulated using 5% w/w of tea tree oil. A number of formulations were prepared at various pH values (5.0, 5.5, 6.0, 6.5, and 7.0). Thermal stability studies showed that the formulations were stable for more than eight months. Agar dilution tests showed MICs of 1.0% v/v S. aureus and S. epidermidis. In the broth dilution test, MBC of the oil for P. acnes was 0.5% v/v. MIC and MBC values were comparable to those of non-formulated tea tree oil, indicating that tea tree oil retained its activity in the above-mentioned formulations. The microbiological evaluation showed that the formulations containing 5% w/w tea tree oil had a maximum effect at pH 5.5.
研究了pH对互叶白千层精油制剂抗菌活性的影响。使用5%（w/w）的茶树油制备了微乳液、脂质体分散体、多重乳液和无菌粘土胶体床。在不同pH值（5.0、5.5、6.0、6.5和7.0）下制备了多种制剂。热稳定性研究表明，这些制剂在八个多月内保持稳定。琼脂稀释试验显示，金黄色葡萄球菌和表皮葡萄球菌的最低抑菌浓度（MIC）为1.0%（v/v）。在肉汤稀释试验中，该油对痤疮丙酸杆菌的最低杀菌浓度（MBC）为0.5%（v/v）。MIC和MBC值与未配制的茶树油相当，表明茶树油在上述制剂中保持了其活性。微生物学评估表明，含有5%（w/w）茶树油的制剂在pH 5.5时效果最佳。
