Raman A, Weir U, Bloomfield S F
Department of Pharmacy, King's College London, UK.
Lett Appl Microbiol. 1995 Oct;21(4):242-5. doi: 10.1111/j.1472-765x.1995.tb01051.x.
Major components of two tea-tree oil samples were identified using thin layer and gas-liquid chromatography (TLC and GLC). Using a TLC-bioautographic technique, the tea-tree oils, terpinen-4-ol, alpha-terpineol and alpha-pinene were found to be active against Staphylococcus aureus, Staph. epidermidis and Propionibacterium acnes whereas cineole was inactive against these organisms. The MIC values of the three active compounds increased in the order alpha-terpineol < terpinen-4-ol < alpha-pinene for all three micro-organisms. MIC values of the tea-tree oils and terpinen-4-ol were lower for P. acnes than for the two staphylococci. This study supports the use of tea-tree oil in the treatment of acne, and demonstrates that terpinen-4-ol is not the sole active constituent of the oil.
利用薄层色谱法和气液色谱法（TLC和GLC）鉴定了两个茶树油样品的主要成分。采用TLC-生物自显影技术，发现茶树油、萜品-4-醇、α-萜品醇和α-蒎烯对金黄色葡萄球菌、表皮葡萄球菌和痤疮丙酸杆菌有活性，而桉叶油素对这些微生物无活性。对于所有三种微生物，这三种活性化合物的最低抑菌浓度（MIC）值按α-萜品醇＜萜品-4-醇＜α-蒎烯的顺序增加。痤疮丙酸杆菌的茶树油和萜品-4-醇的MIC值低于两种葡萄球菌。本研究支持茶树油在痤疮治疗中的应用，并表明萜品-4-醇不是该油的唯一活性成分。
