Jansson Leif, Bodin Birgitta, Carlsson Per-Ola
Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.
Ups J Med Sci. 2005;110(1):57-67. doi: 10.3109/2000-1967-082.
Organ transplantation is associated with changes in graft blood flow, both acutely caused by reperfusion associated phenomena, and chronically due to e.g. denervation. The aim of the study was to investigate regional blood flow early after implantation of a syngeneic pancreas-duodenum transplant in rats, i.e. during reperfusion. Warm ischemia time was 1-2 min and cold ischemia 90 min. Blood flow values were measured with coloured microspheres both 10 and 30 min after implantation in transplanted rats, and at one time point in control rats. A marked decrease in the blood perfusion of the transplanted duodenum compared to the endogenous intestine was seen at both 10 and 30 min. Total graft pancreatic blood flow was increased both 10 and 30 min after implantation, whilst islet blood flow remained unchanged compared to the endogenous gland. We conclude that the blood perfusion of the graft is markedly changed in the immediate post-transplantation period, presumably due to reperfusion. However, islet blood perfusion remains constant, suggesting that islet vasculature is less sensitive to changes induced by the implantation.
器官移植与移植器官血流的变化相关，这种变化在急性情况下是由再灌注相关现象引起的，而在慢性情况下则例如是由于去神经支配。本研究的目的是调查大鼠同种异体胰腺 - 十二指肠移植术后早期，即再灌注期间的局部血流情况。热缺血时间为1 - 2分钟，冷缺血时间为90分钟。在移植大鼠植入后10分钟和30分钟，以及在对照大鼠的一个时间点，用彩色微球测量血流值。与自身肠道相比，移植十二指肠的血流灌注在10分钟和30分钟时均显著降低。植入后10分钟和30分钟时，移植胰腺的总血流均增加，而与自身胰岛相比，胰岛血流保持不变。我们得出结论，移植后即刻移植器官的血流灌注发生了显著变化，推测是由于再灌注所致。然而，胰岛血流灌注保持恒定，这表明胰岛血管系统对植入引起的变化不太敏感。
