Jansson L, Korsgren O, Wahlberg J, Andersson A
Department of Medical Cell Biology, Uppsala University, Sweden.
Transpl Int. 1993 May;6(3):143-7. doi: 10.1007/BF00336357.
The aim of the present study was to evaluate the effects of cyclosporin A (CyA) on the blood perfusion of the transplanted pancreas. For this purpose syngeneic pancreaticoduodenal transplantations were performed in Wistar-Furth rats. After nephrectomy the graft was anastomosed using a nonsuturing cuff technique to the left renal vessels. Beginning 7 days after transplantation and then continuing for 2 weeks, CyA (15 mg/kg body weight) or vehicle was given p.o. once daily, 6 days a week. The serum CyA concentrations were greater than 600 ng/ml at all points in time tested. Intraperitoneal glucose tolerance tests were normal in CyA-treated animals after 12 days, but the pancreatic insulin concentration was decreased to the same extent in the native and transplanted pancreas. A microsphere technique was used to measure the blood perfusion of the pancreaticoduodenal graft, the native pancreas and duodenum, and remaining kidney 14 days after starting the CyA treatment. The renal blood flow was markedly decreased by CyA when compared with the control animals. In rats given vehicle alone, pancreatic, islet, and duodenal blood flows were higher in the graft than in the corresponding native organs. However, in rats given CyA, hyperperfusion of the graft was not observed. We conclude that the administration of CyA prevents the transplantation-induced blood flow increase seen in pancreaticoduodenal grafts of vehicle-treated rats. These observations may reflect graft denervation.
本研究的目的是评估环孢素A（CyA）对移植胰腺血液灌注的影响。为此，在Wistar-Furth大鼠中进行了同基因胰十二指肠移植。肾切除术后，采用非缝合袖套技术将移植物与左肾血管吻合。移植后7天开始，持续2周，口服给予CyA（15mg/kg体重）或赋形剂，每周6天，每天1次。在所有测试时间点，血清CyA浓度均大于600ng/ml。CyA治疗的动物在12天后腹腔内葡萄糖耐量试验正常，但天然胰腺和移植胰腺中的胰岛素浓度均下降到相同程度。在开始CyA治疗14天后，采用微球技术测量胰十二指肠移植物、天然胰腺和十二指肠以及剩余肾脏的血液灌注。与对照动物相比，CyA使肾血流量显著降低。在仅给予赋形剂的大鼠中，移植物中的胰腺、胰岛和十二指肠血流量高于相应的天然器官。然而，在给予CyA的大鼠中，未观察到移植物的血流过度灌注。我们得出结论，给予CyA可防止在给予赋形剂的大鼠胰十二指肠移植物中出现的移植诱导的血流增加。这些观察结果可能反映了移植物去神经支配。
