Tarun Maricar, Rusling James F
Department of Chemistry, 55 North Eagleville Road, University of Connecticut, Storrs, Connecticut 06269-3060, USA.
Anal Chem. 2005 Apr 1;77(7):2056-62. doi: 10.1021/ac048283r.
Neutral hydrolysis and LC-MS/MS analysis of 6-nm-thick DNA-polyion films used in voltammetric genotoxicity screening sensors showed that concentrations of N7-guanine DNA adducts with methyl methanesulfonate and styrene oxide increased with incubation time with the same trends as found for sensor response. Results show that the genotoxicity sensors can be used to estimate relative DNA damage rates for chemical toxicity screening. Neutral thermal hydrolysis provided a relatively clean sample matrix allowing quantitative estimates of nucleobase adducts after several minutes of incubation with damage agents. In addition, an approximate standardization procedure for neutral thermal hydrolysis was developed and validated that avoids need for a pure standard and should be useful in cases where nucleobase adduct standards are unavailable or where their identities are unknown.
用于伏安法遗传毒性筛选传感器的6纳米厚DNA-聚离子膜的中性水解及液相色谱-串联质谱分析表明，与甲磺酸甲酯和氧化苯乙烯形成的N7-鸟嘌呤DNA加合物的浓度随孵育时间增加，其趋势与传感器响应一致。结果表明，遗传毒性传感器可用于估计化学毒性筛选的相对DNA损伤率。中性热水解提供了相对纯净的样品基质，使得在与损伤剂孵育几分钟后能够对核碱基加合物进行定量估计。此外，还开发并验证了一种中性热水解的近似标准化程序，该程序无需纯标准品，在核碱基加合物标准品不可用或其身份未知的情况下应会很有用。
