Tarun Maricar, Bajrami Besnik, Rusling James F
Anal Chem. 2006 Jan 15;78(2):624-7. doi: 10.1021/ac0517996.
Detection of DNA adducts can serve as a basis for genotoxicty screening of new chemicals and drugs. We report here a simple, sensitive procedure for this purpose using films containing DNA and a biocatalyst to mimic the metabolic action of human liver cytochrome P450s. DNA adducts formed from an in-situ-generated toxic metabolite (styrene oxide) were detected at subpicomole levels after neutral thermal hydrolysis of the DNA films and analysis with capillary liquid chromatography with on-line column preconcentration and MS/MS detection. An on-line column switching system allowed for increased sample loading volume and analyte preconcentration. This approach provides an estimate of the relative rate of DNA damage.
DNA加合物的检测可作为新化学品和药物遗传毒性筛选的基础。我们在此报告一种为此目的而设计的简单、灵敏的方法，该方法使用含有DNA的薄膜和一种生物催化剂来模拟人肝细胞色素P450的代谢作用。在对DNA薄膜进行中性热水解并用毛细管液相色谱进行在线柱预富集和MS/MS检测后，可在亚皮摩尔水平检测到由原位生成的有毒代谢物（氧化苯乙烯）形成的DNA加合物。在线柱切换系统可增加样品进样量并实现分析物预富集。这种方法可估算DNA损伤的相对速率。
