Sakagami H, Asano K, Tanuma S, Hatano T, Yoshida T, Okuda T
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Anticancer Res. 1992 Mar-Apr;12(2):377-87.
Various tannins and related compounds were compared for their ability to stimulate the iodination (incorporation of radioactive iodine into an acid-insoluble fraction) of human peripheral blood monocytes. The stimulating activity of most of the monomeric and dimeric hydrolyzable tannins was generally higher than that of the trimeric and tetrameric compounds. Compounds that had dehydrohexahydroxydiphenoyl or chebuloyl groups had considerably less activity than those that had other functional groups (hexahydroxydiphenoyl, valoneoyl, dehydrodigalloyl, isodehydrodigalloyl, lactonized valoneoyl, hellinoyl, euphorbinoyl, dehydroeuphorbinoyl or woodfordinoyl group). The methylated derivative, nonacosa-O-methylcoriariin A, was essentially inactive, suggesting the requirement of a phenolic hydroxyl group. Three condensed tannins ((-)-epicatechin 3-O-gallate (ECG)-dimer, ECG-trimer and ECG-tetramer) significantly stimulated both monocyte iodination and their interleukin-1-like factor production. The results suggest the dependence of stimulation of monocyte iodination by tannins and related polyphenols on molecular weight.
比较了各种单宁及相关化合物刺激人外周血单核细胞碘化作用（将放射性碘掺入酸不溶性部分）的能力。大多数单体和二聚体可水解单宁的刺激活性通常高于三聚体和四聚体化合物。具有脱氢六羟基二苯甲酰基或诃子酰基的化合物的活性明显低于具有其他官能团（六羟基二苯甲酰基、戊二酰基、脱氢双没食子酰基、异脱氢双没食子酰基、内酯化戊二酰基、海林酰基、大戟酰基、脱氢大戟酰基或乌檀酰基）的化合物。甲基化衍生物二十九-O-甲基马桑亭A基本无活性，表明需要酚羟基。三种缩合单宁（（-）-表儿茶素3-O-没食子酸酯（ECG）-二聚体、ECG-三聚体和ECG-四聚体）显著刺激单核细胞碘化及其白细胞介素-1样因子的产生。结果表明，单宁及相关多酚对单核细胞碘化的刺激作用取决于分子量。
