Sakagami H, Asano K, Hara Y, Shimamura T
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
J Leukoc Biol. 1992 May;51(5):478-83. doi: 10.1002/jlb.51.5.478.
(-)Epigallocatechin gallate (EGCg) stimulated iodination of human peripheral blood monocytes, polymorphonuclear cells (PMN), and human promyelocytic leukemic HL-60 cells, dependent on time, dose, and temperature. However, EGCg did not affect iodination of nonadherent peripheral blood mononuclear cells, red blood cells, or 11 other cultured cell lines. Although various immunoregulators such as lipopolysaccharide (LPS), opsonized zymosan, 12-O-tetradecanoylphorbol-13-acetate (TPA) and tumor necrosis factor stimulated PMN iodination to varying degrees, their ability to stimulate monocyte iodination was much lower than that of EGCg. Washout experiments demonstrated that contact with EGCg for less than 60 min irreversibly stimulated PMN and monocyte iodination. EGCg also potently stimulated the production of interleukin-1-like factor by monocytes. The data suggest that EGCg is a strong in vitro stimulant of human phagocytes.
（-）表没食子儿茶素没食子酸酯（EGCg）可刺激人外周血单核细胞、多形核细胞（PMN）以及人早幼粒细胞白血病HL-60细胞的碘化作用，且这种刺激作用依赖于时间、剂量和温度。然而，EGCg并不影响非黏附性外周血单个核细胞、红细胞或其他11种培养细胞系的碘化作用。尽管各种免疫调节剂，如脂多糖（LPS）、调理酵母聚糖、12-O-十四酰佛波醇-13-乙酸酯（TPA）和肿瘤坏死因子，可不同程度地刺激PMN碘化作用，但它们刺激单核细胞碘化的能力远低于EGCg。洗脱实验表明，与EGCg接触少于60分钟会不可逆地刺激PMN和单核细胞的碘化作用。EGCg还能强烈刺激单核细胞产生白细胞介素-1样因子。这些数据表明，EGCg是一种强大的体外人吞噬细胞刺激剂。
