Sakagami H, Hatano T, Yoshida T, Tanuma S, Hata N, Misawa Y, Ishii N, Tsutsumi T, Okuda T
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Anticancer Res. 1990 Nov-Dec;10(6):1523-31.
Tannic acid stimulated the iodination (incorporation of radioactive iodine into an acid-insoluble fraction) of human peripheral blood polymorphonuclear cells (PMN) and human promyelocytic leukemic HL-60 cells, without affecting the iodination of 9 other cultured cell lines. The stimulation of both PMN and HL-60 cells depended on incubation time and temperature, and was significantly suppressed by myeloperoxidase inhibitors. Among chemically defined natural polyphenols, condensed tannins (epicatechin gallate oligomers) and monomeric and oligomeric hydrolyzable tannins potently stimulated PMN iodination, whereas polyphenols of lower molecular weight (gallic acid, alkyl gallates, epicatechin, epicatechin gallate, epigallocatechin, caffeic acid derivatives and licorice flavonoids) had much less activity. Various synthetic polyphenolic compounds structurally unrelated to tannins also stimulated PMN iodination depending upon their molecular weight, but to a slightly lesser extent. The results suggest that the stimulation activity of tannins and related polyphenols might depend more on their molecular weights than the number of hydroxyl groups on each benzene ring in the molecule, or the presence of sugars or hexahydroxydiphenoyl groups.
单宁酸刺激人外周血多形核白细胞（PMN）和人早幼粒细胞白血病HL-60细胞的碘化作用（将放射性碘掺入酸不溶性部分），而不影响其他9种培养细胞系的碘化作用。对PMN和HL-60细胞的刺激作用取决于孵育时间和温度，并被髓过氧化物酶抑制剂显著抑制。在化学定义的天然多酚中，缩合单宁（表儿茶素没食子酸酯低聚物）以及单体和低聚可水解单宁能有效刺激PMN碘化，而分子量较低的多酚（没食子酸、没食子酸烷基酯、表儿茶素、表儿茶素没食子酸酯、表没食子儿茶素、咖啡酸衍生物和甘草黄酮）活性则低得多。各种与单宁结构无关的合成多酚化合物也根据其分子量刺激PMN碘化，但程度稍小。结果表明，单宁和相关多酚的刺激活性可能更多地取决于其分子量，而非分子中每个苯环上羟基的数量，或糖或六羟基二苯甲酰基的存在。
