Department of Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Int Arch Allergy Immunol. 2013;161(3):213-9. doi: 10.1159/000346318. Epub 2013 Mar 15.
Immunoglobulin E (IgE) reactivity to individual allergens among cockroach-allergic patients has revealed wide variability. The aim of this study was to assess the effectiveness of recombinant cockroach allergens for skin testing, and to determine sensitization profiles among cockroach-allergic patients living in Brazil.
Fifty-seven cockroach-allergic patients with asthma and/or rhinitis were recruited. Skin testing with recombinant (r) allergens from Periplaneta americana (rPer a 1 and rPer a 7) and Blattella germanica (rBla g 2, rBla g 4 and rBla g 5) were performed at 10 μg/ml and 5 μg/ml (rPer a 1). IgE antibodies to rPer a 7 and rPer a 1 were quantitated by ELISA.
Of 57 patients tested, 3 (5.3%), 24 (42.1%), 4 (7%), 3 (5.3%) and 4 (7%) showed positive reactions to rPer a 1, rPer a 7, rBla g 2, rBla g 4 and rBla g 5, respectively. Twenty-eight patients (49.1%) had positive tests to at least one allergen. In keeping with skin test results, 31/57 patients (54.4%) and 5/55 patients (9%) had detectable IgE to rPer a 7 and rPer a 1, respectively. Levels of IgE to rPer a 7 were higher in patients with positive tests to rPer a 7 than those with negative tests (geometric mean 13.2 and 1.8 IU/ml, p < 0.05). There was good concordance of results of skin tests and measurements of serum IgE to rPer a 7.
IgE reactivity to rPer a 7 (P. americana tropomyosin) was dominant among patients in Brazil. However, 50% of the patients did not present reactivity to any of the recombinant allergens tested.
对蟑螂过敏患者的个体过敏原免疫球蛋白 E（IgE）反应存在很大差异。本研究旨在评估重组蟑螂过敏原用于皮肤测试的效果，并确定生活在巴西的蟑螂过敏患者的致敏情况。
招募了 57 名患有哮喘和/或鼻炎的蟑螂过敏患者。用美洲大蠊（rPer a 1 和 rPer a 7）和德国小蠊（rBla g 2、rBla g 4 和 rBla g 5）的重组（r）过敏原以 10 μg/ml 和 5 μg/ml（rPer a 1）进行皮肤测试。通过 ELISA 定量检测 rPer a 7 和 rPer a 1 的 IgE 抗体。
在 57 名接受测试的患者中，分别有 3（5.3%）、24（42.1%）、4（7%）、3（5.3%）和 4（7%）对 rPer a 1、rPer a 7、rBla g 2、rBla g 4 和 rBla g 5 呈阳性反应。28 名患者（49.1%）至少对一种过敏原呈阳性反应。与皮肤测试结果一致，57 名患者中有 31 名（54.4%）和 55 名患者中有 5 名（9%）对 rPer a 7 和 rPer a 1 可检测到 IgE。对 rPer a 7 呈阳性反应的患者的 rPer a 7 IgE 水平高于呈阴性反应的患者（几何均数 13.2 和 1.8 IU/ml，p<0.05）。皮肤测试和 rPer a 7 血清 IgE 测量的结果具有良好的一致性。
在巴西患者中，rPer a 7（美洲大蠊肌球蛋白）对 rPer a 7 的 IgE 反应占主导地位。然而，50%的患者对测试的任何重组过敏原均无反应。
