Arcamone Nadia, Lucini Carla, Borzacchiello Giuseppe, Castaldo Luciana, Gargiulo Giuliana, De Girolamo Paolo
Dipartimento di Strutture, Funzioni e Tecnologie Biologiche, Università di Napoli Federico II, Via F. Delpino 1, 1 I-80137 Naples, Italy.
Microsc Res Tech. 2005 Jan 1;66(1):17-24. doi: 10.1002/jemt.20138.
By means of immunochemistry and immunohistochemistry, we investigated in the kidney of freshwater and marine teleostean species for the presence and localization of three neurotrophins: nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin (NT)-3. In both species studied, NGF-like and NT-3-like immunoreactivity were present in the kidney with different distribution patterns, while BDNF-like immunoreactivity was never detected. In goldfish, NGF-like and NT-3-like immunoreactivity were identified extensively in cells along part of the arterial branches adjacent to the afferent arterioles. In scorpion fish, NGF-like and NT-3-like immunoreactive cells were observed both on afferent arterioles and on adjacent secondary branches derived from renal arteries. No immunoreactivity was detected in other renal structures. A staining pattern of immunoreactivity similar to that obtained for NGF and NT-3 was detected utilizing S100 antibody as a juxtaglomerular (JG) cell marker. Double immunolabellings NGF/S100 and NT-3/S100 evidenced the coexistence of neurotrophin-like proteins and S100-like protein in the same immunoreactive cells, thus identifying them as juxtaglomerular cells. Western blot analysis revealed the presence of molecules immunoreactive to NGF and NT-3, whose molecular weights were very similar to those of the corresponding mammalian neurotrophins. These findings extend the presence and distribution of NGF-like and NT-3-like IR in the kidney to teleost species, suggesting a probable participation of these proteins in the renal functions of freshwater and marine teleosts.
通过免疫化学和免疫组织化学方法，我们研究了淡水硬骨鱼和海水硬骨鱼肾脏中三种神经营养因子的存在情况和定位：神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养因子（NT）-3。在所研究的两种鱼类中，肾脏中均存在NGF样和NT-3样免疫反应性，但其分布模式不同，而从未检测到BDNF样免疫反应性。在金鱼中，沿与入球小动脉相邻的部分动脉分支的细胞中广泛发现了NGF样和NT-3样免疫反应性。在鲉鱼中，在入球小动脉和源自肾动脉的相邻二级分支上均观察到了NGF样和NT-3样免疫反应性细胞。在其他肾脏结构中未检测到免疫反应性。利用S100抗体作为球旁（JG）细胞标记物，检测到了与NGF和NT-3相似的免疫反应性染色模式。NGF/S100和NT-3/S100双重免疫标记证明了神经营养因子样蛋白和S100样蛋白在同一免疫反应性细胞中共存，从而将它们鉴定为球旁细胞。蛋白质印迹分析显示存在与NGF和NT-3免疫反应的分子，其分子量与相应的哺乳动物神经营养因子非常相似。这些发现将肾脏中NGF样和NT-3样免疫反应性的存在和分布扩展到硬骨鱼物种，表明这些蛋白质可能参与淡水和海水硬骨鱼的肾脏功能。
