Jones A W, Beylich K M, Bjørneboe A, Ingum J, Mørland J
Department of Alcohol Toxicology, National Laboratory of Forensic Chemistry, University Hospital, Linköping, Sweden.
Clin Chem. 1992 May;38(5):743-7.
We determined the concentrations of ethanol in nearly simultaneous specimens of venous blood (BAC) and end-expired breath (BrAC) after healthy volunteers drank moderate amounts of alcohol. BAC was measured at two laboratories and BrAC was analyzed with two instruments (Intoxilyzer 5000) from the same manufacturer. The mean difference in BAC between laboratories was 0.0105 mg/g (SD 0.0219); 95% of the differences ranged from -0.0333 to 0.0543 mg/g. The mean difference in BrAC between instruments was 0.0153 mg/L (SD 0.0136), and 95% of the differences ranged from -0.0119 to 0.0425 mg/L. The coefficient of variation (CV) between laboratories was 2.9% compared with 4.5% between breath-test instruments. Venous BAC (y) and BrAC (x) were highly correlated (r = 0.978). However, when the Intoxilyzer instruments indicated that BrAC had reached zero, the actual BAC was 0.135 mg/g, according to the average forensic laboratory reports. The Intoxilyzer 5000 breath analyzers used in this study seem to have a constant analytical bias.
我们测定了健康志愿者饮用适量酒精后几乎同时采集的静脉血样本（血液酒精浓度，BAC）和终末呼气样本（呼气酒精浓度，BrAC）中的乙醇浓度。血液酒精浓度在两个实验室进行测量，呼气酒精浓度使用同一制造商生产的两台仪器（Intoxilyzer 5000）进行分析。实验室之间血液酒精浓度的平均差异为0.0105 mg/g（标准差0.0219）；95%的差异范围在-0.0333至0.0543 mg/g之间。仪器之间呼气酒精浓度的平均差异为0.0153 mg/L（标准差0.0136），95%的差异范围在-0.0119至0.0425 mg/L之间。实验室之间的变异系数（CV）为2.9%，而呼气测试仪器之间为4.5%。静脉血血液酒精浓度（y）和呼气酒精浓度（x）高度相关（r = 0.978）。然而，根据法医实验室的平均报告，当Intoxilyzer仪器显示呼气酒精浓度达到零时，实际血液酒精浓度为0.135 mg/g。本研究中使用的Intoxilyzer 5000呼气分析仪似乎存在恒定的分析偏差。
