Van Dyck E, Rossau R, Duhamel M, Behets F, Laga M, Nzila M, Bygdeman S, Van Heuverswijn H, Piot P
Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium.
Genitourin Med. 1992 Apr;68(2):111-6. doi: 10.1136/sti.68.2.111.
To determine the in vitro antimicrobial susceptibility of gonococcal strains isolated in 1988 among female prostitutes in Kinshasa, Zaire and to characterise strains with high level tetracycline resistance.
Minimal inhibitory concentrations of 8 antimicrobials were measured by agar dilution technique. Plasmid-profiles and serovars were determined.
Two hundred and thirteen strains of Neisseria gonorrhoeae were tested of which 59% were beta-lactamase producers and an additional 21% showed intermediate or chromosomal resistance to penicillin (MIC = 0.5-8 mg/l). Eleven percent of the strains were resistant to the combination sulfamethoxazole-trimethoprim (MIC greater than 8 mg/l) and 57% of the isolates showed decreased susceptibility to thiamphenicol (MIC = 1-4 mg/l). All strains were sensitive to spectinomycin, norfloxacin and ceftriaxone and moderately sensitive to kanamycin. Chromosomal resistance to tetracycline was observed in 45% of strains (MIC = 2-8 mg/l). Ten percent were highly resistant to tetracycline (TRNG, MIC = 16-128 mg/l) and were shown to carry a plasmid borne Tet M determinant; such strains were not found in Kinshasa in 1985. TRNG belonged to 4 different serovars, which were also the dominant serovars in non-TRNG.
These findings illustrate the high frequency of multiresistant gonococci in Zaire and suggest that high level tetracycline resistant strains of N. gonorrhoeae have become endemic in Central Africa.
测定1988年在扎伊尔金沙萨从女性性工作者中分离出的淋球菌菌株的体外抗菌药敏情况，并对具有高水平四环素耐药性的菌株进行特征分析。
采用琼脂稀释技术测定8种抗菌药物的最低抑菌浓度。确定质粒图谱和血清型。
检测了213株淋病奈瑟菌，其中59%为β-内酰胺酶产生菌，另有21%对青霉素表现出中介或染色体介导的耐药性（MIC = 0.5 - 8 mg/l）。11%的菌株对复方磺胺甲恶唑耐药（MIC大于8 mg/l），57%的分离株对甲砜霉素敏感性降低（MIC = 1 - 4 mg/l）。所有菌株对壮观霉素、诺氟沙星和头孢曲松敏感，对卡那霉素中度敏感。45%的菌株观察到对四环素的染色体介导耐药（MIC = 2 - 8 mg/l）。10%对四环素高度耐药（TRNG，MIC = 16 - 128 mg/l），并显示携带质粒介导的Tet M决定簇；1985年在金沙萨未发现此类菌株。TRNG属于4种不同的血清型，它们也是非TRNG中的优势血清型。
这些发现表明扎伊尔多重耐药淋球菌的频率很高，并提示淋病奈瑟菌高水平四环素耐药菌株已在中非成为地方病。
