Bhalla P, Sethi K, Reddy B S, Mathur M D
Maulana Azad Medical College and Associated Lok Nayak Hospital, Department of Microbiology, New Delhi, India.
Sex Transm Infect. 1998 Jun;74(3):210-2. doi: 10.1136/sti.74.3.210.
To determine the antibiotic susceptibility and plasmid profile of all Neisseria gonorrhoeae strains (PPNG and non-PPNG) isolated from May 1995 to March 1996 in Lok Nayak Hospital, New Delhi, India.
The agar plate dilution method was used to determine the minimum inhibitory concentration of five antimicrobials including norfloxacin and ceftriaxone which are most commonly used for treatment of gonorrhoea in Delhi. Isolates were screened for production of penicillinase by paper acidometric method and plasmid analysis of PPNG and non-PPNG was carried out by agarose gel electrophoresis.
50 consecutive isolates of N gonorrhoeae were studied, 8% among them were found to be PPNG while 28% were highly resistant to tetracycline (TRNG). Reduced susceptibility to norfloxacin (MIC > or = 1 microgram/ml) was observed in 12% of all isolates. All PPNG harboured the 4.4 MDa beta lactamase plasmid along with the 25.2 MDa tetracycline resistance plasmid. Norfloxacin resistance (MIC > or = 1 microgram/ml) was present in 28.5% of TRNG but only in 5.5% of the other gonococcal isolates.
Results of this study clearly demonstrate that antibiotic resistant gonococcal strains of different clones are frequently found in New Delhi. Continued surveillance of susceptibility to currently prescribed antimicrobials and epidemiological studies are essential to prevent treatment failures leading to further spread of resistant strains.
确定1995年5月至1996年3月在印度新德里洛克·奈亚克医院分离出的所有淋病奈瑟菌菌株（产青霉素酶淋病奈瑟菌和非产青霉素酶淋病奈瑟菌）的抗生素敏感性和质粒图谱。
采用琼脂平板稀释法测定包括诺氟沙星和头孢曲松在内的五种抗菌药物的最低抑菌浓度，这两种药物是德里最常用于治疗淋病的药物。通过纸酸度测定法筛选分离株的青霉素酶产生情况，并通过琼脂糖凝胶电泳对产青霉素酶淋病奈瑟菌和非产青霉素酶淋病奈瑟菌进行质粒分析。
对50株连续的淋病奈瑟菌分离株进行了研究，其中8%被发现是产青霉素酶淋病奈瑟菌，而28%对四环素高度耐药（耐四环素淋病奈瑟菌）。在所有分离株中，12%对诺氟沙星的敏感性降低（最低抑菌浓度≥1微克/毫升）。所有产青霉素酶淋病奈瑟菌都携带4.4兆道尔顿的β-内酰胺酶质粒以及25.2兆道尔顿的四环素耐药质粒。耐四环素淋病奈瑟菌中有28.5%对诺氟沙星耐药（最低抑菌浓度≥1微克/毫升），而其他淋病奈瑟菌分离株中只有5.5%耐药。
本研究结果清楚地表明，在新德里经常发现不同克隆的抗生素耐药淋病奈瑟菌菌株。持续监测目前规定的抗菌药物的敏感性以及进行流行病学研究对于防止治疗失败导致耐药菌株进一步传播至关重要。
