具有超亮且光稳定发射特性的水溶性杂化纳米团簇：一种用于生物成像的新工具。
Water-soluble hybrid nanoclusters with extra bright and photostable emissions: a new tool for biological imaging.
作者信息
Makarava Natallia, Parfenov Alexander, Baskakov Ilia V
机构信息
Medical Biotechnology Center, University of Maryland Biotechnology Institute, Baltimore, Maryland 21201, USA.
出版信息
Biophys J. 2005 Jul;89(1):572-80. doi: 10.1529/biophysj.104.049627. Epub 2005 Apr 15.
Abstract
We report the generation of a previously unknown class of water-soluble organic-inorganic hybrid nanoclusters composed of silver and thioflavin T with remarkable fluorescent properties. These hybrid nanoclusters display an extra bright fluorescence in aqueous solutions without any detectable photobleaching. Furthermore, the fluorescent nanoclusters can be generated in situ by sensitized photoreduction of Ag+. Organic-inorganic nanoclusters have remarkable advantages over the known classes of fluorescent probes for the development of ultrasensitive biological assays, cell imaging, or studies of single molecules. As an example of a practical biological application, imaging of amyloid fibrils produced from recombinant mammalian prion proteins and nonprion proteins using hybrid nanoclusters is presented.
摘要
我们报道了一类由银和硫黄素T组成的、具有显著荧光特性的前所未知的水溶性有机-无机杂化纳米团簇的生成。这些杂化纳米团簇在水溶液中显示出极强的荧光，且无任何可检测到的光漂白现象。此外，荧光纳米团簇可通过Ag⁺的敏化光还原原位生成。与已知的荧光探针类别相比，有机-无机纳米团簇在开发超灵敏生物测定、细胞成像或单分子研究方面具有显著优势。作为实际生物应用的一个例子，展示了使用杂化纳米团簇对重组哺乳动物朊病毒蛋白和非朊病毒蛋白产生的淀粉样纤维进行成像。
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