Wang Qiong, Zhuang Guang-lun, Zhou Can-quan, Li Jie, Zhong Yi-ping, Xu Yan-wen, Zhang Ming-fan, Deng Ming-fen
Reproductive Center, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Zhonghua Fu Chan Ke Za Zhi. 2005 Feb;40(2):112-5.
To find out the expression of soluble human leukocyte antigen G (sHLA-G) and its relationship to the cleavage embryo development.
One hundred and seventy-seven day 3 cleavage embryos were detected for sHLA-G by immunohistochemistry.
sHLA-G was detected in 57.1% cleavage embryos. The positive rate of sHLA-G in cleavage embryos developed from dipronucleate fertilized eggs was 66.2% (90/136), that developed from tripronucleate fertilized eggs was 26.8% (11/41). There was significant difference between these two groups (P < 0.01). The positive rate of sHLA-G in the grade 1 cleavage embryos developed from dipronucleate fertilized eggs was 64.3% (18/28), that in the grade 2 cleavage embryos developed from dipronucleate fertilized eggs was 91.7% (66/72), that in the grade 3 cleavage embryos developed from dipronucleate fertilized eggs was 16.7% (6/36), there were significant differences between these different embryo grades (P < 0.01), and the intensity of sHLA-G had negative relationship with the embryo grades (r = -0.503). The positive rate of sHLA-G in the first class cleavage embryos developed from tripronucleate fertilized eggs was 88.9% (32/36), that from dipronucleate fertilized eggs was 64.3% (18/28). There was significant difference in these two groups (P < 0.01). The intensity of sHLA-G in cleavage embryos developed from tripronucleate fertilized eggs was higher than that from dipronucleate fertilized eggs. The positive rate of sHLA-G in the cleavage embryos developed from dipronucleate fertilized eggs whose cell number was less than 4 was 56.7% (34/60), that from dipronucleate fertilized eggs whose cell number ranged from 5 to 6 was 67.9% (36/53), and that from dipronucleate fertilized eggs whose cell number ranged from 7 to 8 was 87.0% (20/23). There were no significant differences in these three groups. The intensity of sHLA-G had no significant difference between embryos with different cell number (P > 0.05), but it had relationship with the cell number (r = 0.267).
Cleavage embryos express sHLA-G which is related with the embryo development.
探讨可溶性人类白细胞抗原G（sHLA-G）的表达及其与卵裂期胚胎发育的关系。
采用免疫组织化学方法检测177枚第3天的卵裂期胚胎中的sHLA-G。
57.1%的卵裂期胚胎检测到sHLA-G。来自双原核受精卵的卵裂期胚胎中sHLA-G阳性率为66.2%（90/136），来自三原核受精卵的为26.8%（11/41），两组间差异有统计学意义（P<0.01）。来自双原核受精卵的1级卵裂期胚胎中sHLA-G阳性率为64.3%（18/28），2级为91.7%（66/72），3级为16.7%（6/36），不同胚胎级别间差异有统计学意义（P<0.01），且sHLA-G表达强度与胚胎级别呈负相关（r=-0.503）。来自三原核受精卵的1类卵裂期胚胎中sHLA-G阳性率为88.9%（32/36），来自双原核受精卵的为64.3%（18/28），两组间差异有统计学意义（P<0.01）。来自三原核受精卵的卵裂期胚胎中sHLA-G表达强度高于来自双原核受精卵的。来自双原核受精卵、细胞数小于4的卵裂期胚胎中sHLA-G阳性率为56.7%（34/60），细胞数为5～6的为67.9%（36/53），细胞数为7～8的为87.0%（20/23），三组间差异无统计学意义。不同细胞数的胚胎中sHLA-G表达强度差异无统计学意义（P>0.05），但与细胞数有关（r=0.267）。
卵裂期胚胎表达sHLA-G，其与胚胎发育有关。
