Sargent Ian, Swales Anna, Ledee Nathalie, Kozma Noemi, Tabiasco Julie, Le Bouteiller Philippe
Oxford Fertility Unit, Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Oxford, UK.
J Reprod Immunol. 2007 Oct;75(2):128-32. doi: 10.1016/j.jri.2007.03.005. Epub 2007 May 4.
A number of reports have demonstrated that sHLA-G can be detected in the culture medium of human IVF embryos and that levels correlate with the potential of an embryo to implant. This has aroused considerable interest in the IVF field. If sHLA-G can be used as a non-invasive marker of embryo quality, it will facilitate selection of the best embryos to transfer to the mother and thereby increase IVF pregnancy rates. However, there have been concerns about some aspects of these studies, including the sensitivity of the sHLA-G ELISAs used, the IVF culture conditions and the levels of sHLA-G which have been reported. A recent study by Sageshima et al. [J. Reprod. Immunol. 75, 11-22, 2007] attempts to address some of these concerns. However, despite using a sensitive ELISA, they were unable to detect sHLA-G in 111 embryo culture supernatants, or sHLA-G secretion by less than 10,000 sHLA-G transfected cells. They concluded that it is not possible to measure sHLA-G production by human embryos. This study has highlighted technical differences between IVF culture techniques and sHLA-G ELISAs that are currently confounding the system. Further collaboration between the research groups involved is required to establish robust reproducible systems that function identically in all laboratories.
多项报告表明，在人类体外受精（IVF）胚胎的培养基中可检测到可溶性人类白细胞抗原G（sHLA - G），且其水平与胚胎着床潜力相关。这在IVF领域引起了相当大的关注。如果sHLA - G可作为胚胎质量的非侵入性标志物，将有助于选择最佳胚胎移植给母体，从而提高IVF妊娠率。然而，这些研究的某些方面引发了担忧，包括所用sHLA - G酶联免疫吸附测定（ELISA）的灵敏度、IVF培养条件以及所报道的sHLA - G水平。Sageshima等人[《生殖免疫学杂志》75卷，第11 - 22页，2007年]最近的一项研究试图解决其中一些问题。然而，尽管使用了灵敏的ELISA，他们仍无法在111份胚胎培养上清液中检测到sHLA - G，也无法检测到少于10,000个转染sHLA - G的细胞分泌的sHLA - G。他们得出结论，无法测量人类胚胎产生的sHLA - G。这项研究突出了IVF培养技术与目前使该系统混乱的sHLA - G ELISA之间的技术差异。参与研究的各小组之间需要进一步合作，以建立在所有实验室中功能相同的可靠可重复系统。
