Baydas Giyasettin, Ozveren Faik, Tuzcu Mehmet, Yasar Abdullah
Department of Physiology, College of Medicine, Firat University, Elazig 23119, Turkey.
Eur J Pharmacol. 2005 Apr 11;512(2-3):181-7. doi: 10.1016/j.ejphar.2005.02.038.
Thinner containing 60-70% toluene is a neurotoxic mixture, which is widely used as an aromatic industrial solvent. This product has been shown to cause functional and structural changes in the central nervous system. Thinner generates reactive oxygen species and the toxic effects relating to these reactants. We have investigated the effect of exposure to high concentrations (3000 ppm) of thinner for 45 days (1 h/day) on cognitive function and the levels of neural cell adhesion molecules (NCAM) and lipid peroxidation products in the hippocampus, cortex and cerebellum of rats. Thinner exposure caused a significant increase in lipid peroxidation products (malondialdehyde and 4-hydroxyalkenals) in all brain regions. Levels of NCAM 140 and NCAM 180 kDa were significantly decreased in the hippocampus and cortex of the thinner-exposed group. Furthermore, thinner-exposed rats showed cognitive deficits in passive avoidance and Morris water maze tasks. These cognitive deficits may be due to both elevated oxidative stress and changes in synaptic plasticity. Almost all studied parameters were reversed in animals which were allowed to recover from thinner inhalation, suggesting that the effects of exposure to thinner, at least for 1 h/day for 45 days, are reversible.
含有60 - 70%甲苯的稀释剂是一种神经毒性混合物，被广泛用作芳香族工业溶剂。该产品已被证明会导致中枢神经系统发生功能和结构变化。稀释剂会产生活性氧以及与这些反应物相关的毒性作用。我们研究了大鼠在45天内每天暴露于高浓度（3000 ppm）稀释剂1小时对其认知功能以及海马体、皮层和小脑中神经细胞黏附分子（NCAM）水平和脂质过氧化产物的影响。接触稀释剂导致所有脑区的脂质过氧化产物（丙二醛和4 - 羟基烯醛）显著增加。在接触稀释剂组的海马体和皮层中，NCAM 140和180 kDa的水平显著降低。此外，接触稀释剂的大鼠在被动回避和莫里斯水迷宫任务中表现出认知缺陷。这些认知缺陷可能是由于氧化应激升高和突触可塑性变化所致。几乎所有研究参数在从吸入稀释剂中恢复的动物中都得到了逆转，这表明至少在每天暴露1小时、持续45天的情况下，接触稀释剂的影响是可逆的。
