Bailey Dalan, Banyard Ashley, Dash Pradyot, Ozkul Aykut, Barrett Tom
Pirbright Laboratory, Institute for Animal Health, Ash Road, Woking, Surrey GU24 ONF, UK.
Virus Res. 2005 Jun;110(1-2):119-24. doi: 10.1016/j.virusres.2005.01.013.
Peste des petits ruminants virus (PPRV) causes an acute febrile illness in small ruminant species, mostly sheep and goats. PPRV is a member of the Morbillivirus genus which includes measles, rinderpest (cattle plague), canine distemper, phocine distemper and the morbilliviruses found in whales, porpoises and dolphins. Full length genome sequences for these morbilliviruses are available and reverse genetic rescue systems have been developed for the viruses of terrestrial mammals, with the exception of PPRV. This paper presents the first published full length genome sequence for PPRV. The genome was found to be consistent with the rule-of-six and open reading frames (ORFs) were identified that encoded the eight proteins characteristic of morbilliviruses. At the nucleotide (nt) level, the full length genome of PPRV was most similar to that of rinderpest, the other ruminant morbillivirus. However, at the protein level five of the six structural proteins and the V protein showed a greater similarity to the dolphin morbillivirus (DMV) while only the C and L proteins showed a high relationship to rinderpest.
小反刍兽疫病毒（PPRV）可引起小反刍动物（主要是绵羊和山羊）的急性发热性疾病。PPRV属于麻疹病毒属，该属还包括麻疹病毒、牛瘟病毒、犬瘟热病毒、海豹瘟热病毒以及在鲸鱼、鼠海豚和海豚中发现的麻疹病毒。这些麻疹病毒的全长基因组序列已可获取，并且除PPRV外，已为陆生哺乳动物的病毒开发了反向遗传拯救系统。本文公布了首个PPRV全长基因组序列。发现该基因组符合六规则，并鉴定出了编码麻疹病毒特征性八种蛋白质的开放阅读框（ORF）。在核苷酸（nt）水平上，PPRV的全长基因组与另一种反刍动物麻疹病毒——牛瘟病毒的基因组最为相似。然而，在蛋白质水平上，六种结构蛋白中的五种以及V蛋白与海豚麻疹病毒（DMV）显示出更高的相似性，而只有C蛋白和L蛋白与牛瘟病毒有高度相关性。
