Duftner Christina, Seiler Rüdiger, Klein-Weigel Peter, Göbel Heike, Goldberger Christian, Ihling Christian, Fraedrich Gustav, Schirmer Michael
Department of Internal Medicine, Innsbruck Medical University, Innsbruck, Austria.
Arterioscler Thromb Vasc Biol. 2005 Jul;25(7):1347-52. doi: 10.1161/01.ATV.0000167520.41436.c0. Epub 2005 Apr 21.
To assess the possible role of proinflammatory CD28- T cells in abdominal aortic aneurysms (AAAs). Animal studies and human tissue studies suggest a role for interferon (IFN)-gamma-producing T cells in the development and progression of AAAs.
Fluorescence-activated cells sorter analysis of peripheral blood samples and measurement of AAA size using sonography were performed in 101 AAA patients and 38 healthy controls. Peripheral percentages of CD28- T cells of the CD3+CD4+ and the CD3+CD8+ were enriched in AAA patients with 7.8+/-8.8% and 41.9+/-15.7% compared with healthy controls with 2.2+/-6.1% and 24.9+/-15.5%, respectively (P=0.002 and P<0.001, respectively). Both CD4+CD28- and CD8+CD28- T cells produced large amounts of IFN-[gamma] and perforin. Patients with small AAAs (<4 cm) showed higher peripheral levels of CD4+CD28- T cells than those with larger AAAs (P=0.025). Immunohistological examinations revealed 39.1+/-17.2% CD4+CD28- and 44.0+/-13.8% CD8+CD28- in AAA tissue specimens with inflammatory infiltratestes.
IFN-gamma- and perforin-producing CD28- T cells are present in the periphery and the vessel wall of a majority of AAAs. This observation in humans favors the concept of a T cell-mediated pathophysiology of AAAs, especially during the early development of AAAs.
评估促炎CD28 - T细胞在腹主动脉瘤（AAA）中的可能作用。动物研究和人体组织研究表明，产生干扰素（IFN）-γ的T细胞在AAA的发生和发展中起作用。
对101例AAA患者和38例健康对照者进行外周血样本的荧光激活细胞分选分析，并使用超声测量AAA大小。与健康对照者（分别为2.2±6.1%和24.9±15.5%）相比，AAA患者CD3 + CD4 +和CD3 + CD8 +的CD28 - T细胞外周百分比增加，分别为7.8±8.8%和41.9±15.7%（P分别为0.002和P<0.001）。CD4 + CD28 -和CD8 + CD28 - T细胞均产生大量IFN -γ和穿孔素。小AAA（<4 cm）患者的外周CD4 + CD28 - T细胞水平高于大AAA患者（P = 0.025）。免疫组织学检查显示，AAA组织标本中有炎症浸润的CD4 + CD28 -细胞占39.1±17.2%，CD8 + CD28 -细胞占44.0±13.8%。
大多数AAA的外周和血管壁中存在产生IFN -γ和穿孔素的CD28 - T细胞。在人类中的这一观察结果支持AAA的T细胞介导的病理生理学概念，尤其是在AAA的早期发展过程中。
