Abad J P, Carmena M, Baars S, Saunders R D, Glover D M, Ludeña P, Sentis C, Tyler-Smith C, Villasante A
Centro de Biología Molecular (CSIC-UAM), Spain.
Proc Natl Acad Sci U S A. 1992 May 15;89(10):4663-7. doi: 10.1073/pnas.89.10.4663.
To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G+C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.
为了鉴定来自着丝粒区域的序列，我们构建了一个黑腹果蝇酵母人工染色体（YAC）文库，并用从X染色体衍生的小染色体Dp(1;f)1187中纯化的DNA对其进行筛选。我们描述了通过这种方式分离出的一个克隆的结构。这个YAC在结构上不稳定，包含串联重复的富含G+C的11聚体和12聚体单元，我们将其称为十二聚体卫星序列。该卫星序列的大部分位于常染色体的着丝粒附近。在远至拟南芥和智人的生物体基因组中都发现了交叉杂交序列。
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