Department of Experimental Biology, Genetics, University of Jaén. Paraje las Lagunillas sn., 23071 Jaén, Spain.
Evolutionary Genetic Section, Faculty of Science, University of the Republic, Iguá 4225, Montevideo 11400, Uruguay.
Int J Mol Sci. 2021 Jun 3;22(11):6052. doi: 10.3390/ijms22116052.
The triatomine is the main vector of Chagas disease in countries such as Colombia and Venezuela, and the first kissing bug whose genome has been sequenced and assembled. In the repetitive genome fraction (repeatome) of this species, the transposable elements represented 19% of genome, being mostly DNA transposon (Class II elements). However, scarce information has been published regarding another important repeated DNA fraction, the satellite DNA (satDNA), or satellitome. Here, we offer, for the first time, extended data about satellite DNA families in the genome using bioinformatics pipeline based on low-coverage sequencing data. The satellitome of represents 8% of the total genome and it is composed by 39 satDNA families, including four satDNA families that are shared with , as well as telomeric (TTAGG) and (GATA) repeats, also present in the genome. Only three of them exceed 1% of the genome. Chromosomal hybridization with these satDNA probes showed dispersed signals over the euchromatin of all chromosomes, both in autosomes and sex chromosomes. Moreover, clustering analysis revealed that most abundant satDNA families configured several superclusters, indicating that satellitome is complex and that the four most abundant satDNA families are composed by different subfamilies. Additionally, transcription of satDNA families was analyzed in different tissues, showing that 33 out of 39 satDNA families are transcribed in four different patterns of expression across samples.
锥蝽是哥伦比亚和委内瑞拉等国恰加斯病的主要传播媒介，也是第一个完成基因组测序和组装的接吻虫。在该物种的重复基因组部分（重复组）中，转座元件占基因组的 19%，主要是 DNA 转座子（第二类元件）。然而，关于另一个重要的重复 DNA 部分，卫星 DNA（satDNA）或 satellitome，发表的信息很少。在这里，我们首次使用基于低覆盖测序数据的生物信息学管道，提供了关于 基因组中卫星 DNA 家族的扩展数据。 基因组的 satellitome 占总基因组的 8%，由 39 个 satDNA 家族组成，其中包括与 共享的四个 satDNA 家族，以及端粒（TTAGG）和（GATA）重复序列，这些重复序列也存在于 基因组中。只有其中三个超过了基因组的 1%。用这些 satDNA 探针进行染色体杂交显示，所有染色体的常染色质上都有分散的信号，无论是在常染色体还是性染色体上。此外，聚类分析表明，最丰富的 satDNA 家族构成了几个超级簇，表明 satellitome 很复杂，四个最丰富的 satDNA 家族由不同的亚家族组成。此外，还分析了 satDNA 家族在不同组织中的转录情况，结果表明，39 个 satDNA 家族中有 33 个家族在 4 个不同的表达模式中在 4 个样本中表达。
