Brake S R, Marsik F J, Rein M R
Am J Med Technol. 1982 Jan;48(1):43-6.
A four-hour micromedia method which detects enzymes formed by bacteria for the degradion of carbohydrates was compared to the utilization of carbohydrates was compared to the utilization of carbohydrates in cystine tyrpticase agar (CTA) for the identification of Neisseria gonorrhoeae and Neisseria meningitidis. This rapid micromedia method (RMM) correlated 100% with the utilization of carbohydrates in CTA. Identification of N. gonorrhoeae by RMM was compared to the identification achieved by a commercially available coagglutination method and a fluorescent antibody (FA) technique. Of 144 isolates identified as N. gonorrhoeae by RMM, 122 (84.7%) were identified by coagglutination and 141 (97.9%) were identified by FA as N. gonorrhoeae. Five (13%) of 40 isolates identified as N. meningitidis by RMM were identified as N. gonorrhoeae by coagglutination while eleven (28%) were identified as N. gonorrhoeae by the FA technique. One (14%) and four (57%) of seven isolates identified as Neisseria species were identified as N. gonorrhoeae by coagglutination and the FA technique respectively. The rapid micromedia method was found to be a quick, sensitive, specific and economic way of identifying N. gonorrhoeae and N. meningitidis.
将一种检测细菌形成的用于降解碳水化合物的酶的四小时微量培养基方法，与在胱氨酸胰蛋白酶琼脂（CTA）中利用碳水化合物来鉴定淋病奈瑟菌和脑膜炎奈瑟菌的方法进行了比较。这种快速微量培养基方法（RMM）与CTA中碳水化合物的利用情况的相关性为100%。将通过RMM对淋病奈瑟菌的鉴定结果与通过市售的协同凝集方法和荧光抗体（FA）技术所获得的鉴定结果进行了比较。在通过RMM鉴定为淋病奈瑟菌的144株分离株中，有122株（84.7%）通过协同凝集被鉴定为淋病奈瑟菌，141株（97.9%）通过FA被鉴定为淋病奈瑟菌。在通过RMM鉴定为脑膜炎奈瑟菌的40株分离株中，有5株（13%）通过协同凝集被鉴定为淋病奈瑟菌，而11株（28%）通过FA技术被鉴定为淋病奈瑟菌。在鉴定为奈瑟菌属的7株分离株中，分别有1株（14%）和4株（57%）通过协同凝集和FA技术被鉴定为淋病奈瑟菌。结果发现，快速微量培养基方法是一种快速、灵敏、特异且经济的鉴定淋病奈瑟菌和脑膜炎奈瑟菌的方法。
