Maniar A C
Health Lab Sci. 1977 Jan;14(1):26-9.
Clinical isolates of Neisseria gonorrhoeae from Manitoba were tested for their carbohydrate degradation activity in Cystine Trypticase Agar (C.T.A) medium, Mueller-Hinton Agar and guinea pig serum agar. Each isolate was tested using 2 carbohydrates (e.g. glucose and maltose) in the above three media. Out of 661 isolates tested, only 80% were positively identified in C.T.A. medium. Mueller-Hinton agar allowed 88% identification while guinea pig serum agar yielded 100% identification. In a second series of experiments, 102 cultures of N. gonorrhoeae were used to compare Flynn & Waitkins medium with guinea pig serum agar. Only 91 of these were identified with Flynn and Waitkins medium while guinea pig serum agar identified all the 102 isolates. Guinea pig serum provides adequate growth of fastidious N. gonorrhoeae essential for detecting specific enzymes. Since guinea pig serum does not contain maltase activity, it does not interfere with the biochemical activities tested. Guinea pig serum agar is easy to prepare, does not require a heavy inoculum and gives definite color change in the medium.
对来自曼尼托巴省的淋病奈瑟菌临床分离株，在胱氨酸胰蛋白酶琼脂（C.T.A）培养基、穆勒-欣顿琼脂和豚鼠血清琼脂中测试其碳水化合物降解活性。每个分离株在上述三种培养基中使用两种碳水化合物（如葡萄糖和麦芽糖）进行测试。在测试的661个分离株中，只有80%在C.T.A培养基中得到阳性鉴定。穆勒-欣顿琼脂的鉴定率为88%，而豚鼠血清琼脂的鉴定率为100%。在第二系列实验中，使用102株淋病奈瑟菌培养物将弗林和沃特金斯培养基与豚鼠血清琼脂进行比较。弗林和沃特金斯培养基仅鉴定出其中的91株，而豚鼠血清琼脂鉴定出了所有102个分离株。豚鼠血清能为检测特定酶所必需的苛求淋病奈瑟菌提供充足的生长条件。由于豚鼠血清不含有麦芽糖酶活性，因此不会干扰所测试的生化活性。豚鼠血清琼脂易于制备，不需要大量接种物，并且能使培养基产生明确的颜色变化。
