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1
Evaluation of methods for the rapid identification of Neisseria gonorrhoeae in a routine clinical laboratory.常规临床实验室中淋病奈瑟菌快速鉴定方法的评估
J Clin Microbiol. 1976 Jul;4(1):19-21. doi: 10.1128/jcm.4.1.19-21.1976.
2
A comparison of three fermentation methods for the confirmation of Neisseria gonorrhoeae.三种用于淋病奈瑟菌确证的发酵方法的比较
Health Lab Sci. 1976 Jan;13(1):54-8.
3
Rapid fermentation confirmation of Neisseria gonorrhoeae.淋病奈瑟菌的快速发酵鉴定
Appl Microbiol. 1973 Apr;25(4):550-2. doi: 10.1128/am.25.4.550-552.1973.
4
Evaluation of the RIM system and Gono Gen test for identification of Neisseria gonorrhoeae from clinical specimens.评估RIM系统和淋菌基因检测用于从临床标本中鉴定淋病奈瑟菌的效果。
Diagn Microbiol Infect Dis. 1985 Mar;3(2):125-30. doi: 10.1016/0732-8893(85)90021-5.
5
Rapid carbohydrate utilization test for the identification of Neisseria gonorrhoeae.用于鉴定淋病奈瑟菌的快速碳水化合物利用试验。
Br J Vener Dis. 1976 Jun;52(3):172-5. doi: 10.1136/sti.52.3.172.
6
Comparison of GonoGen, GonoGen II, and MicroTrak direct fluorescent-antibody test with carbohydrate fermentation for confirmation of culture isolates of Neisseria gonorrhoeae.将淋病奈瑟菌培养分离株进行确证时，GonoGen、GonoGen II和MicroTrak直接荧光抗体检测与碳水化合物发酵法的比较。
J Clin Microbiol. 1995 Feb;33(2):474-6. doi: 10.1128/jcm.33.2.474-476.1995.
7
A simple carbohydrate fermentation test for identification of the pathogenic Neisseria.一种用于鉴定致病性奈瑟菌的简单碳水化合物发酵试验。
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8
[A culture method suitable in general practice for the diagnosis of gonorrhea].[一种适用于淋病诊断的一般临床实践的培养方法]
Arch Hyg Bakteriol. 1969 Oct;153(5):473-7.
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Fluorescent monoclonal antibody compared with carbohydrate utilization for rapid identification of Neisseria gonorrhoeae.荧光单克隆抗体与碳水化合物利用法用于淋病奈瑟菌快速鉴定的比较
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Simple disk-plate method for the biochemical confirmation of pathogenic Neisseria.用于致病性奈瑟菌生化鉴定的简易圆盘平板法
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引用本文的文献

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J Clin Microbiol. 1993 Sep;31(9):2535-7. doi: 10.1128/jcm.31.9.2535-2537.1993.
2
Comparison of GonoGen, GonoGen II, and MicroTrak direct fluorescent-antibody test with carbohydrate fermentation for confirmation of culture isolates of Neisseria gonorrhoeae.将淋病奈瑟菌培养分离株进行确证时，GonoGen、GonoGen II和MicroTrak直接荧光抗体检测与碳水化合物发酵法的比较。
J Clin Microbiol. 1995 Feb;33(2):474-6. doi: 10.1128/jcm.33.2.474-476.1995.
3
Rapid identification of pathogenic species of Neisseria by carbohydrate degradation tests. Importance of glucose in media used for preparation of inocula.通过碳水化合物降解试验快速鉴定奈瑟菌的致病菌种。葡萄糖在用于制备接种物的培养基中的重要性。
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Evaluation of rapid carbohydrate degradation tests for identification of pathogenic Neisseria.用于鉴定致病性奈瑟菌的快速碳水化合物降解试验的评估
J Clin Microbiol. 1980 Apr;11(4):394-7. doi: 10.1128/jcm.11.4.394-397.1980.
6
Evaluation of the phadebact gonococcus test, a coagglutination procedure for confirmation of Neisseria gonorrhoeae.对“法德巴克淋球菌试验”（一种用于确诊淋病奈瑟菌的协同凝集试验方法）的评估。
J Clin Microbiol. 1980 Feb;11(2):153-6. doi: 10.1128/jcm.11.2.153-156.1980.
7
Rapid confirmatory identification of Neisseria gonorrhoeae with lectins and chromogenic substrates.利用凝集素和显色底物快速确证性鉴定淋病奈瑟菌
J Clin Microbiol. 1984 Mar;19(3):380-2. doi: 10.1128/jcm.19.3.380-382.1984.
8
Identification of pathogenic Neisseria species with the RapID NH system.使用RapID NH系统鉴定致病性奈瑟菌属菌种。
J Clin Microbiol. 1983 Mar;17(3):400-4. doi: 10.1128/jcm.17.3.400-404.1983.
9
Use of the API NeIdent system for identification of pathogenic Neisseria spp. and Branhamella catarrhalis.使用API NeIdent系统鉴定致病性奈瑟菌属和卡他布兰汉菌。
J Clin Microbiol. 1984 Mar;19(3):338-41. doi: 10.1128/jcm.19.3.338-341.1984.
10
Difficulties in differentiating Neisseria cinerea from Neisseria gonorrhoeae in rapid systems used for identifying pathogenic Neisseria species.在用于鉴定致病性奈瑟菌属物种的快速检测系统中，区分灰色奈瑟菌和淋病奈瑟菌存在困难。
J Clin Microbiol. 1985 Nov;22(5):731-4. doi: 10.1128/jcm.22.5.731-734.1985.

本文引用的文献

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A NEW RAPID IMMUNOFLUORESCENT STAINING TECHNIQUE FOR IDENTIFICATION OF TREPONEMA PALLIDUM AND NEISSERIA GONORRHOEAE.一种用于鉴定梅毒螺旋体和淋病奈瑟菌的新型快速免疫荧光染色技术。
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2
Possible effects of antibiotic treatment on the senstivity and growth requirements of Neisseria gonorrhoea.抗生素治疗对淋病奈瑟菌敏感性和生长需求的可能影响。
Bull World Health Organ. 1961;24(3):333-42.
3
A simple fermentation medium for Neisseria gonorrhoeae.一种用于淋病奈瑟菌的简单发酵培养基。
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4
Combined use of fluorescent antibody technique and culture on selective medium for the identification of Neisseria gonorrhoeae.荧光抗体技术与选择性培养基培养相结合用于淋病奈瑟菌的鉴定。
Acta Pathol Microbiol Scand. 1969;76(2):279-87. doi: 10.1111/j.1699-0463.1969.tb03258.x.
5
Improved medium selective for cultivation of N. gonorrhoeae and N. meningitidis.改良的用于培养淋病奈瑟菌和脑膜炎奈瑟菌的培养基。
Public Health Rep (1896). 1966 Jun;81(6):559-62.
6
Further studies with a selective medium for cultivating Neisseria gonorrhoeae.使用用于培养淋病奈瑟菌的选择性培养基的进一步研究。
Br J Vener Dis. 1965 Sep;41(3):199-201. doi: 10.1136/sti.41.3.199.
7
Rapid fermentation confirmation of Neisseria gonorrhoeae.淋病奈瑟菌的快速发酵鉴定
Appl Microbiol. 1973 Apr;25(4):550-2. doi: 10.1128/am.25.4.550-552.1973.
8
Modification of the rapid fermentation test for Neisseria gonorrhoeae.淋病奈瑟菌快速发酵试验的改良
Appl Microbiol. 1974 Jun;27(6):1027-30. doi: 10.1128/am.27.6.1027-1030.1974.
9
Fluroescent antibody technique for identification of presumptively positive gonococcal cultures.用于鉴定疑似阳性淋病奈瑟菌培养物的荧光抗体技术。
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10
Carbohydrate fermentation plate medium for confirmation of Neisseria species.用于鉴定奈瑟菌属菌种的碳水化合物发酵平板培养基。
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常规临床实验室中淋病奈瑟菌快速鉴定方法的评估

Evaluation of methods for the rapid identification of Neisseria gonorrhoeae in a routine clinical laboratory.

作者信息

Pollock H M

出版信息

J Clin Microbiol. 1976 Jul;4(1):19-21. doi: 10.1128/jcm.4.1.19-21.1976.

DOI:10.1128/jcm.4.1.19-21.1976
PMID:821966
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274382/
Abstract

Of 78 isolates of Neisseria gonorrhoeae, 21 failed to grow and produce acid in unsupplemented cystine-Trypticase agar (CTA); whereas positive reactions were obtained by using serum-supplemented CTA and fluorescent antibody (FA). An additional 290 strains of Neisseria were evaluated by FA and by a rapid carbohydrate degradation technique (RF). There was agreement between the two methods 92% of the time on the initial trial and 99% of the time with repeats on discrepancies. The RF and FA tests provided rapid and reliable identification of N. gonorrhoeae, alleviating the problems of CTA due to lack of growth and need for overnight incubation.

摘要

在78株淋病奈瑟菌中，有21株在未添加血清的胱氨酸胰蛋白酶琼脂（CTA）中无法生长和产酸；而使用添加血清的CTA和荧光抗体（FA）可获得阳性反应。另外290株奈瑟菌通过FA和快速碳水化合物降解技术（RF）进行评估。在初次试验中，两种方法的一致性为92%，在对差异进行重复试验时，一致性为99%。RF和FA试验能够快速、可靠地鉴定淋病奈瑟菌，解决了因缺乏生长以及需要过夜培养而导致的CTA相关问题。