常规临床实验室中淋病奈瑟菌快速鉴定方法的评估
Evaluation of methods for the rapid identification of Neisseria gonorrhoeae in a routine clinical laboratory.
作者信息
Pollock H M
出版信息
J Clin Microbiol. 1976 Jul;4(1):19-21. doi: 10.1128/jcm.4.1.19-21.1976.
Abstract
Of 78 isolates of Neisseria gonorrhoeae, 21 failed to grow and produce acid in unsupplemented cystine-Trypticase agar (CTA); whereas positive reactions were obtained by using serum-supplemented CTA and fluorescent antibody (FA). An additional 290 strains of Neisseria were evaluated by FA and by a rapid carbohydrate degradation technique (RF). There was agreement between the two methods 92% of the time on the initial trial and 99% of the time with repeats on discrepancies. The RF and FA tests provided rapid and reliable identification of N. gonorrhoeae, alleviating the problems of CTA due to lack of growth and need for overnight incubation.
摘要
在78株淋病奈瑟菌中，有21株在未添加血清的胱氨酸胰蛋白酶琼脂（CTA）中无法生长和产酸；而使用添加血清的CTA和荧光抗体（FA）可获得阳性反应。另外290株奈瑟菌通过FA和快速碳水化合物降解技术（RF）进行评估。在初次试验中，两种方法的一致性为92%，在对差异进行重复试验时，一致性为99%。RF和FA试验能够快速、可靠地鉴定淋病奈瑟菌，解决了因缺乏生长以及需要过夜培养而导致的CTA相关问题。
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