Holme S, Snyder E, Heaton A, Keegan T, Napychank P
Department of Pathology, Eastern Virginia Medical School, Norfolk.
Transfusion. 1992 May;32(4):328-33. doi: 10.1046/j.1537-2995.1992.32492263446.x.
The effect of cotton wool filtration of apheresis platelet concentrates (PCs) on platelet viability and complement activation was evaluated by two laboratories. PCs were prepared by automated (Lab A, n = 5) or manual (Lab B, n = 5) apheresis. After storage for 1 day, the PC was filtered through cotton wool before transfusion on one occasion and, on the other occasion, filtered through a standard screen filter before transfusion to the same donor. Five paired studies were performed by each laboratory. Except for a small, but significant reduction in mean platelet size, from 7.3 +/- 1.1 to 6.6 +/- 0.9 microns 3, after cotton wool filtration, no effect of filtration on various tests of in vitro platelet function and morphologic integrity was found. As demonstrated by autologous radiolabeled studies, no effect of cotton wool filtration on platelet viability was found by Laboratory B, while Laboratory A found a slight increase in the percentage of recovery from 59 +/- 4 to 68 +/- 13 percent, and a small reduction in survival, from 8.2 +/- 0.9 to 7.7 +/- 0.5 days after cotton wool filtration (p less than 0.05). Cotton wool filtration was associated with a slight increase in C3a levels found in manual apheresis PCs. Neither laboratory found any effect of cotton wool filtration per se on the recipients' white cell (WBC) counts or C3a and C5a levels after transfusion.(ABSTRACT TRUNCATED AT 250 WORDS)
两个实验室评估了棉絮过滤单采血小板浓缩物（PCs）对血小板活力和补体激活的影响。PCs通过自动（实验室A，n = 5）或手工（实验室B，n = 5）单采制备。储存1天后，一份PC在输注前通过棉絮过滤，另一份在输注给同一供者前通过标准筛网过滤器过滤。每个实验室进行了5项配对研究。除了棉絮过滤后平均血小板大小有小幅但显著的减小，从7.3±1.1微米³降至6.6±0.9微米³外，未发现过滤对体外血小板功能和形态完整性的各种测试有影响。自体放射性标记研究表明，实验室B未发现棉絮过滤对血小板活力有影响，而实验室A发现棉絮过滤后回收率百分比略有增加，从59±4%增至68±13%，存活率略有降低，从8.2±0.9天降至7.7±0.5天（p＜0.05）。棉絮过滤与手工单采PCs中C3a水平略有升高有关。两个实验室均未发现棉絮过滤本身对受者输血后的白细胞（WBC）计数或C3a和C5a水平有任何影响。（摘要截取自250字）
