Grabherr R, Strasser P, Van Etten J L
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Virology. 1992 Jun;188(2):721-31. doi: 10.1016/0042-6822(92)90527-v.
The deduced amino acid sequences of two eukaryotic chlorella virus (PBCV-1 and NY-2A) DNA polymerases are 90% identical and contain amino acid motifs typical of alpha-like (Family B) DNA polymerases. The open reading frames of both PBCV-1 and NY-2A DNA polymerases are interrupted by an identically located, small (101 or 86 nucleotides, respectively) intron that resembles eukaryotic nuclear-spliced messenger RNA introns. This discovery suggests that chlorella virus replication has a nuclear phase.
两种真核小球藻病毒（PBCV-1和NY-2A）DNA聚合酶的推导氨基酸序列有90%的同一性，且包含α类（B家族）DNA聚合酶典型的氨基酸基序。PBCV-1和NY-2A DNA聚合酶的开放阅读框都被一个位置相同的小内含子（分别为101或86个核苷酸）打断，该内含子类似于真核细胞核剪接信使RNA内含子。这一发现表明小球藻病毒复制有一个核阶段。
