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猪胰岛素样生长因子结合蛋白1基因定位于18号染色体及通过PCR-RFLP检测第2内含子中的多态性

Assignment of the gene for porcine insulin-like growth factor binding protein 1 to chromosome 18 and detection of polymorphisms in intron 2 by PCR-RFLP.

作者信息

Inage-Miyake Yuko, Shimanuki Shin-ichi, Itoh Tomohito, Murakami Yasuto, Kimura Masahiro, Suzuki Hideaki, Miyake Masashi, Toki Daisuke, Uenishi Hirohide, Awata Takashi, Hamasima Noriyuki

机构信息

Society for Techno-innovation of Agriculture, Forestry and Fisheries Institute, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Ibaraki, Japan.

出版信息

Biochem Genet. 2005 Feb;43(1-2):79-85. doi: 10.1007/s10528-005-1069-1.

DOI:10.1007/s10528-005-1069-1
PMID:15859522
Abstract

We have obtained a partial cDNA and three BAC clones for the porcine insulin-like growth factor binding protein 1 gene (IGFBP-1). Results of fluorescence in situ and radiation hybrid (RH) mapping assigned this gene to porcine chromosome (SSC) 18q24-qter. We found two types of polymerase chain reaction-restriction-fragment-length polymorphisms (PCR-RFLP) in intron 2 by using FokI and AluI.

摘要

我们已经获得了猪胰岛素样生长因子结合蛋白1基因（IGFBP - 1）的部分cDNA和三个BAC克隆。荧光原位杂交和辐射杂种（RH）定位结果将该基因定位于猪18号染色体（SSC）18q24 - qter区域。我们利用FokI和AluI在内含子2中发现了两种聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）。

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