Thomsen P D, Qvist H, Marklund L, Andersson L, Sjöström H, Norén O
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Mamm Genome. 1993;4(10):604-7. doi: 10.1007/BF00361393.
A porcine 2-kb partial dipeptidylpeptidase IV (DPP4, EC 3.4.14.5) cDNA clone and a porcine 16-kb genomic fragment containing parts of the DPP4 gene were isolated, characterized, and used as probes to map the DPP4 gene to pig Chr (Chr) 15q21 by fluorescence in situ hybridization. A two-allele RFLP was revealed for the DPP4 gene. This polymorphism was utilized in a linkage test against the erythrocyte antigen G (EAG), previously assigned to Chr 15, and the microsatellite S0088, which is linked to EAG. The linkage analyses revealed significant evidence for linkage confirming the assignment of DPP4 to Chr 15.
分离出一个猪的2kb部分二肽基肽酶IV（DPP4，EC 3.4.14.5）cDNA克隆以及一个包含DPP4基因部分片段的16kb猪基因组片段，对其进行表征，并用作探针通过荧光原位杂交将DPP4基因定位到猪的15号染色体（Chr）15q21上。发现DPP4基因存在双等位基因限制性片段长度多态性（RFLP）。这种多态性被用于与先前定位到15号染色体的红细胞抗原G（EAG）以及与EAG连锁的微卫星S0088进行连锁测试。连锁分析揭示了显著的连锁证据，证实了DPP4位于15号染色体上。
