高速逆流色谱法从元宝枫中分离槲皮素-3-O-L-鼠李糖苷

Isolation of quercetin-3-O-L-rhamnoside from Acer truncatum Bunge by high-speed counter-current chromatography.

作者信息

Ma Xiaofeng, Tian Weixi, Wu Linhuan, Cao Xueli, Ito Yoichiro

机构信息

Department of Biology, Graduate School of Chinese Academy of Sciences, Beijing 10049, China.

出版信息

J Chromatogr A. 2005 Apr 8;1070(1-2):211-4. doi: 10.1016/j.chroma.2005.02.052.

DOI:10.1016/j.chroma.2005.02.052

PMID:15861807

Abstract

Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of quercetin-3-O-L-rhamnoside from the ethyl acetate extract of the leaves of Acer truncatum Bunge using a two-phase-system composed of ethyl acetate-ethanol-water at a volume ratio of 5:1:5 (v/v/v). In a single operation, 41.9mg of quercetin-3-O-L-rhamnoside was obtained from 366mg of the crude extract. High-performance liquid chromatography (HPLC) analyses of the CCC fraction revealed that the purity of quercetin-3-O-L-rhamnoside was over 96%. Its structure was identified by MS, 1H NMR and 13C NMR. Quercetin-3-O-L-rhamnoside was obtained from this plant for the first time.

摘要

制备型高速逆流色谱法（HSCCC）成功用于从元宝枫叶乙酸乙酯提取物中分离纯化槲皮素-3-O-L-鼠李糖苷，采用体积比为5:1:5（v/v/v）的乙酸乙酯-乙醇-水两相系统。单次操作中，从366mg粗提取物中获得了41.9mg槲皮素-3-O-L-鼠李糖苷。对CCC馏分的高效液相色谱（HPLC）分析表明，槲皮素-3-O-L-鼠李糖苷的纯度超过96%。其结构通过质谱、1H核磁共振和13C核磁共振进行了鉴定。槲皮素-3-O-L-鼠李糖苷首次从该植物中获得。