Le Gall Maud, Quillien Laurence, Guéguen Jacques, Rogniaux Hélène, Sève Bernard
INRA, Unité de Recherche sur les Protéines Végétales et leurs Interactions (URPVI), 44072 Nantes Cedex 03.
J Nutr. 2005 May;135(5):1215-22. doi: 10.1093/jn/135.5.1215.
Ileal flows and the endogenous or dietary origin of soluble proteins present in ileal digesta were determined in pigs fed diets containing different pea cultivars (Solara, Madria and Eiffel) and micro-ground peas (c.v. Solara). Ileal digesta proteins were analyzed by electrophoresis and densitometry analysis and were identified by LC-MS-MS spectrometry and immunoblotting. The ileal flows of proteins differed (P < 0.1) among the 3 pea cultivars; the flow in pigs fed the Madria-containing diet was higher than that of pigs fed the Eiffel- and Solara-containing diets. The flow was reduced by micro-grinding the peas. The true digestibility of pea proteins and the endogenous losses were not correlated. However, at this intestinal level, protein losses were essentially of endogenous origin (enzymes, antibodies), and from the partly digested pea albumin fraction. Pea lectin and albumin PA1b were totally resistant to gastric and small intestinal digestion and a minor resistant peptide from pea albumin PA2 was detectable. In contrast, the storage proteins, legumin and vicilin, were not detectable by antibodies or by LC-MS-MS.
对饲喂含有不同豌豆品种（索拉拉、马德里亚和埃菲尔）及微粉碎豌豆（品种索拉拉）日粮的猪，测定了回肠蛋白质流量以及回肠食糜中可溶性蛋白质的内源性或日粮来源。通过电泳和光密度分析对回肠食糜蛋白质进行分析，并通过液相色谱-串联质谱（LC-MS-MS）和免疫印迹法进行鉴定。3个豌豆品种间回肠蛋白质流量存在差异（P < 0.1）；饲喂含马德里亚日粮的猪的蛋白质流量高于饲喂含埃菲尔和索拉拉日粮的猪。微粉碎豌豆可降低蛋白质流量。豌豆蛋白质的真消化率与内源性损失不相关。然而，在这个肠道水平，蛋白质损失主要源于内源性（酶、抗体）以及部分消化的豌豆白蛋白部分。豌豆凝集素和白蛋白PA1b对胃和小肠消化完全有抗性，并且可检测到来自豌豆白蛋白PA2的少量抗性肽。相比之下，通过抗体或LC-MS-MS无法检测到贮藏蛋白豆球蛋白和豌豆球蛋白。
