Vlcková V, Dúhová V, Svidová S, Farkassová A, Kamasová S, Vlcek D, Kogan G, Rauko P, Miadoková E
Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic.
Cell Biol Toxicol. 2004 Nov;20(6):325-32. doi: 10.1007/s10565-004-0089-7.
Antimutagenic, anticlastogenic, and bioprotective effect of polysaccharide glucomannan (GM) isolated from Candida utilis was evaluated in four model test systems. The antimutagenic effect of GM against 9-aminoacridine (9-AA)- and sodium azide (NaN3)-induced mutagenicity was revealed in the Salmonella typhimurium strains TA97 and TA100, respectively. GM showed anticlastogenic effect against N-nitroso-N'-methylurea (NMU) induced chromosome aberrations in the Vicia sativa assay. The bioprotective effect of GM co-treated with methyl-methane-sulphonate (MMS) was also established in Chlamydomonas reinhardtii repair deficient strains uvs10 and uvs14. The statistically significant antimutagenic potential of GM was not proved against 4-nitro-quinoline-1-oxide (4-NQO)-induced mutagenicity in Saccharomyces cerevisiae D7 assay. It may be due to bioprotectivity of alpha-mannan and beta-glucan, which are integral part of S. cerevisiae cell walls. Due to the good water solubility, low molecular weight (30 kDa), antimutagenic/anticlastogenic, and bioprotective activity against chemical compounds differing in mode of action, GM appears to be a promising natural protective (antimutagenic) agent.
从产朊假丝酵母中分离得到的葡甘露聚糖（GM）的抗诱变、抗断裂和生物保护作用在四个模型测试系统中进行了评估。GM对鼠伤寒沙门氏菌TA97和TA100菌株中分别由9-氨基吖啶（9-AA）和叠氮化钠（NaN3）诱导的诱变性具有抗诱变作用。在蚕豆试验中，GM对N-亚硝基-N'-甲基脲（NMU）诱导的染色体畸变显示出抗断裂作用。在莱茵衣藻修复缺陷菌株uvs10和uvs14中也证实了GM与甲磺酸甲酯（MMS）共同处理时的生物保护作用。在酿酒酵母D7试验中，未证明GM对4-硝基喹啉-1-氧化物（4-NQO）诱导的诱变性具有统计学上显著的抗诱变潜力。这可能是由于α-甘露聚糖和β-葡聚糖的生物保护作用，它们是酿酒酵母细胞壁的组成部分。由于良好的水溶性、低分子量（30 kDa）、抗诱变/抗断裂以及对作用方式不同的化合物具有生物保护活性，GM似乎是一种有前途的天然保护（抗诱变）剂。
