Algire J E, Srikandakumar A, Guilbault L A, Downey B R
Department of Animal Science, McGill University, Ste. Anne de Bellevue, Québec.
Can J Vet Res. 1992 Jan;56(1):67-9.
Indomethacin (INDO, n = 5) or vehicle (CONTROL, n = 4) was injected into superovulated heifers at 48 and 60 h following a luteolytic cloprostenol injection (0 h). One heifer from each group was ovariectomized (OVX) at 48, 56, 64 and 72 h. The fifth heifer of the INDO group was OVX at 80 h. Blood samples were collected at 0 h, every 2 h between 37 and 47 h, and at the time of each OVX to monitor plasma progesterone (P4) and luteinizing hormone (LH) concentrations. Following each OVX, the number and size of follicles were recorded and the incidence of ovulation determined. Follicular fluid (FF) was aspirated from follicles greater than or equal to 8 mm to determine the concentration of prostaglandins E2 (PGE2) and F2 alpha (PGF2 alpha). The highest PG concentrations were measured in both groups at 24-25 h following the preovulatory LH surge and the PGF2 alpha concentration at this time was significantly greater (p less than 0.01) in the CONTROL group compared to the INDO group. By 35-36 h after the LH surge, 75% (25/34) of the CONTROL follicles had ovulated, whereas there were no ovulations (0/50) on either ovary of the INDO treated heifer. These preliminary results suggest that the preovulatory rise of PGs in FF, particularly PGF2 alpha, is essential for ovulation and that suppression of this rise with indomethacin will inhibit ovulation in heifers.
在注射溶黄体的氯前列醇（0小时）后48小时和60小时，向超排的小母牛注射吲哚美辛（INDO，n = 5）或赋形剂（对照组，n = 4）。每组中的一头小母牛在48、56、64和72小时进行卵巢切除（OVX）。吲哚美辛组的第五头小母牛在80小时进行卵巢切除。在0小时、37至47小时之间每2小时以及每次卵巢切除时采集血样，以监测血浆孕酮（P4）和促黄体生成素（LH）浓度。每次卵巢切除后，记录卵泡的数量和大小，并确定排卵发生率。从直径大于或等于8毫米的卵泡中抽吸卵泡液（FF），以测定前列腺素E2（PGE2）和F2α（PGF2α）的浓度。在排卵前促黄体生成素高峰后24至25小时，两组中均测得最高的前列腺素浓度，此时对照组的PGF2α浓度比吲哚美辛组显著更高（p < 0.01）。促黄体生成素高峰后35至36小时，对照组75%（25/34）的卵泡已排卵，而吲哚美辛处理的小母牛两侧卵巢均未排卵（0/50）。这些初步结果表明，卵泡液中前列腺素的排卵前升高，尤其是PGF2α，对排卵至关重要，并且用吲哚美辛抑制这种升高将抑制小母牛排卵。
