Hobel Cédric F V, Marteinsson Viggó T, Hreggvidsson Gudmundur O, Kristjánsson Jakob K
Prokaria Ltd., Gylfaflöt 5, IS-112 Reykjavik, Iceland.
Appl Environ Microbiol. 2005 May;71(5):2771-6. doi: 10.1128/AEM.71.5.2771-2776.2005.
The microbial diversity of intertidal hot springs on the seashore of northwest Iceland was examined by combining directed in situ enrichments, artificial support colonization, and mat sampling. Analysis of 16S rRNA genes revealed the presence of clones related to both marine and terrestrial, thermophilic, mesophilic, and psychrophilic microorganisms scattered among 11 bacterial divisions. No archaea were found. The species composition of the enrichments was affected by the length of the hot periods experienced at low tide and was very different from those found in the biomass. A total of 36 chitinase genes were detected by molecular screening of the samples with degenerate primers for glycoside hydrolase family 18. The chitinase gene diversity was at least twofold higher in the enrichment samples than in the controls, indicating that a much higher diversity of hydrolytic genes can be accessed with this approach.
通过结合定向原位富集、人工支持定殖和垫料采样，对冰岛西北部海岸潮间带温泉的微生物多样性进行了研究。对16S rRNA基因的分析表明，在11个细菌门类中存在与海洋和陆地、嗜热、嗜温和嗜冷微生物相关的克隆。未发现古菌。富集物的物种组成受低潮期高温持续时间的影响，与生物量中的物种组成有很大不同。用糖苷水解酶家族18的简并引物对样品进行分子筛选，共检测到36个几丁质酶基因。富集样品中的几丁质酶基因多样性至少是对照样品的两倍，这表明通过这种方法可以获得更高多样性的水解基因。
