肌浆网Ca2+激活ATP酶的旋转运动及寡聚体结构证据
Rotational motion and evidence for oligomeric structures of sarcoplasmic reticulum Ca2+-activated ATPase.
作者信息
Hoffmann W, Sarzala M G, Chapman D
出版信息
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3860-4. doi: 10.1073/pnas.76.8.3860.
Abstract
The rotational motion of the sarcoplasmic reticulum Ca2+-activated ATPase (ATP phosphohydrolase, EC 3.6.1.3) has been investigated by measuring the decay of laser flash-induced dichroism with the covalently attached triplet probe eosin isothiocyanate. The Arrhenius plot for rotational mobility indicates two discontinuities at approximately 15 degrees C and approximately 35 degrees C. The experimental data are rationalized in terms of a sudden conformeric change in the ATPase at 15 degrees C and a temperature-dependent equilibrium existing between the conformationally altered ATPase and oligomeric forms of it in the temperature range 15-35 degrees C. The enzymatic activity, as indicated by a discontinuity in the Arrhenius plot for the rate of ATP hydrolysis, appears to be sensitive only to the change at 15 degrees C. There is a strong correlation between the activation energy below 15 degrees C for rotational motion (33.6 +/- 2.2 kcal/mol) and enzymatic activity (34 +/- 4 kcal/mol).
摘要
通过使用共价连接的三重态探针异硫氰酸伊红测量激光闪光诱导的二色性衰减，研究了肌浆网Ca2 + 激活的ATP酶（ATP磷酸水解酶，EC 3.6.1.3）的旋转运动。旋转迁移率的阿累尼乌斯图表明在约15℃和约35℃处有两个不连续点。根据ATP酶在15℃时的突然构象变化以及在15 - 35℃温度范围内构象改变的ATP酶与其寡聚形式之间存在的温度依赖性平衡，对实验数据进行了合理解释。如ATP水解速率的阿累尼乌斯图中的不连续点所示，酶活性似乎仅对15℃时的变化敏感。15℃以下旋转运动的活化能（33.6 +/- 2.2千卡/摩尔）与酶活性（34 +/- 4千卡/摩尔）之间存在很强的相关性。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
3
Control of muscle contraction.肌肉收缩的控制
Q Rev Biophys. 1969 Nov;2(4):351-84. doi: 10.1017/s0033583500001190.
4
Rotational diffusion of rhodopsin in the visual receptor membrane.视紫红质在视觉受体膜中的旋转扩散。
Nat New Biol. 1972 Mar 15;236(63):39-43. doi: 10.1038/newbio236039a0.
5
Spectroscopic technique for studying protein rotation in membranes.用于研究膜中蛋白质旋转的光谱技术。
Nat New Biol. 1973 Oct 24;245(147):249-51. doi: 10.1038/newbio245249a0.
6
The effect of anti-ATPase antibodies upon the Ca++ transport of sarcoplasmic reticulum.抗ATP酶抗体对肌浆网Ca++转运的影响。
Biochem Biophys Res Commun. 1974 Sep 9;60(1):382-9. doi: 10.1016/0006-291x(74)90216-2.
7
Activation energies of the ATPase activity of sarcoplasmic reticulum.
Biochem Biophys Res Commun. 1974 Jun 18;58(4):897-904. doi: 10.1016/s0006-291x(74)80228-7.
8
Temperature-induced transitions of function and structure in sarcoplasmic reticulum membranes.肌质网膜中温度诱导的功能与结构转变
J Mol Biol. 1973 Dec 25;81(4):483-504. doi: 10.1016/0022-2836(73)90518-4.
9
The effect of delipidation on the adenosine triphosphatase of sarcoplasmic reticulum. Electron microscopy and physical properties.脱脂对肌浆网三磷酸腺苷酶的影响。电子显微镜检查及物理性质
Eur J Biochem. 1974 Feb 15;42(1):183-93. doi: 10.1111/j.1432-1033.1974.tb03328.x.
10
Phase transitions and fluidity characteristics of lipids and cell membranes.脂质与细胞膜的相变及流动性特征
Q Rev Biophys. 1975 May;8(2):185-235. doi: 10.1017/s0033583500001797.
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